Details

Autor(en) / Beteiligte

• Wei, Wei
• Lu, Long
• Bian, Xiao‐Hua
• Li, Qing‐Tian
• Han, Jia‐Qi
• Tao, Jian‐Jun
• Yin, Cui‐Cui
• Lai, Yong‐Cai
• Li, Wei
• Bi, Ying‐Dong
• Man, Wei‐Qun
• Chen, Shou‐Yi
• Zhang, Jin‐Song
• Zhang, Wan‐Ke

Titel

Zinc‐finger protein GmZF351 improves both salt and drought stress tolerance in soybean

Ist Teil von

• Journal of integrative plant biology, 2023-07, Vol.65 (7), p.1636-1650

Ort / Verlag

China (Republic : 1949- ): Wiley Subscription Services, Inc

Erscheinungsjahr

2023

Link zum Volltext

Quelle

Wiley Blackwell Single Titles

Beschreibungen/Notizen

• ABSTRACT Abiotic stress is one of the most important factors reducing soybean yield. It is essential to identify regulatory factors contributing to stress responses. A previous study found that the tandem CCCH zinc‐finger protein GmZF351 is an oil level regulator. In this study, we discovered that the GmZF351 gene is induced by stress and that the overexpression of GmZF351 confers stress tolerance to transgenic soybean. GmZF351 directly regulates the expression of GmCIPK9 and GmSnRK, leading to stomata closing, by binding to their promoter regions, which carry two CT(G/C)(T/A)AA elements. Stress induction of GmZF351 is mediated through reduction in the H3K27me3 level at the GmZF351 locus. Two JMJ30‐demethylase‐like genes, GmJMJ30‐1 and GmJMJ30‐2, are involved in this demethylation process. Overexpression of GmJMJ30‐1/2 in transgenic hairy roots enhances GmZF351 expression mediated by histone demethylation and confers stress tolerance to soybean. Yield‐related agronomic traits were evaluated in stable GmZF351‐transgenic plants under mild drought stress conditions. Our study reveals a new mode of GmJMJ30‐GmZF351 action in stress tolerance, in addition to that of GmZF351 in oil accumulation. Manipulation of the components in this pathway is expected to improve soybean traits and adaptation under unfavorable environments. The soybean zinc finger protein GmZF351 regulates oil levels and mainly works in seeds. However, the histone demethylase GmJMJ30‐1/2 activates GmZF351 in leaves and roots to improve stress tolerance by mechanisms that differ from the mechanisms acting in seeds.