Details

Autor(en) / Beteiligte

• 蔡培强 汤逊 林月秋 Oudega Martin 孙广运 徐林 阳运康 周田华

Titel

The experimental study of genetic engineering human neural stem cells mediated by lentivirus to express multigene

Ist Teil von

• Chinese journal of traumatology, 2006-02, Vol.9 (1), p.43-49

Ort / Verlag

China: Department of Orthopaedics, the First People's Hospital of Yibin, Yibin 644000, China%Department of Orthopaedics, Kunming General Hospital of Chendu Military Command, Kunming 650032, China%The Miami Project to Cure Paralysis, University of Miami School of Medicine, Miami, FL 33101, USA%Kunming Institute of Zoology, The Chinese Academy of Science, Kunming 650000, China

Erscheinungsjahr

2006

Quelle

MEDLINE

Beschreibungen/Notizen

• Objective： To explore the feasibility to construct genetic engineering human neural stem cells （hNSCs） mediated by lentivirus to express multigene in order to provide a graft source for further studies of spinal cord injury （SCI）. Methods： Human neural stem cells from the brain cortex of human abortus were isolated and cultured, then gene was modified by lentivirus to express both green fluorescence protein （ GFP ） and rat neurotrophin-3 （NT-3）; the transgenic expression was detected by the methods of fluorescence microscope, dorsal root ganglion of fetal rats and slot blot. Results ： Genetic engineering hNSCs were successfully constructed. All of the genetic engineering hNSCs which expressed bright green fluorescence were observed under the fluorescence microscope. The conditioned medium of transgenic hNSCs could induce neurite flourishing outgrowth from dorsal root ganglion （DRG）. The genetic engineering hNSCs expressed high level NT-3 which could be detected by using slot blot. Conclusions ： Genetic engineering hNSCs mediated by lentivirus can be constructed to express multigene successfully.