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The experimental study of genetic engineering human neural stem cells mediated by lentivirus to express multigene
Ist Teil von
Chinese journal of traumatology, 2006-02, Vol.9 (1), p.43-49
Ort / Verlag
China: Department of Orthopaedics, the First People's Hospital of Yibin, Yibin 644000, China%Department of Orthopaedics, Kunming General Hospital of Chendu Military Command, Kunming 650032, China%The Miami Project to Cure Paralysis, University of Miami School of Medicine, Miami, FL 33101, USA%Kunming Institute of Zoology, The Chinese Academy of Science, Kunming 650000, China
Erscheinungsjahr
2006
Quelle
MEDLINE
Beschreibungen/Notizen
Objective: To explore the feasibility to construct genetic engineering human neural stem cells (hNSCs) mediated by lentivirus to express multigene in order to provide a graft source for further studies of spinal cord injury (SCI). Methods: Human neural stem cells from the brain cortex of human abortus were isolated and cultured, then gene was modified by lentivirus to express both green fluorescence protein ( GFP ) and rat neurotrophin-3 (NT-3); the transgenic expression was detected by the methods of fluorescence microscope, dorsal root ganglion of fetal rats and slot blot. Results : Genetic engineering hNSCs were successfully constructed. All of the genetic engineering hNSCs which expressed bright green fluorescence were observed under the fluorescence microscope. The conditioned medium of transgenic hNSCs could induce neurite flourishing outgrowth from dorsal root ganglion (DRG). The genetic engineering hNSCs expressed high level NT-3 which could be detected by using slot blot. Conclusions : Genetic engineering hNSCs mediated by lentivirus can be constructed to express multigene successfully.