Details

Autor(en) / Beteiligte

• Ma, Lu-Lu
• Liu, Wei
• Huang, Yu-Guang
• Yang, Nan
• Zuo, Ping-Ping

Titel

Analgesic effect of gabapentin in a rat model for chronic constrictive injury

Ist Teil von

• Chinese medical journal, 2011-12, Vol.124 (24), p.4304-4309

Ort / Verlag

China: Department of Anesthesiology, Peking Union Medical College Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, Beijing 100710, China%Department of Pharmacology, Basic Science Institute, Chinese Academy of Medical Sciences, Peking Union Medical College,Beijing 100710, China

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• Background Gabapentin has been widely and successfully used in the clinic for many neuropathic pain syndromes since last decade, however its analgesic mechanisms are still elusive. Our study was to investigate whether Ca^2＋/calmodulin-dependent protein kinase Ⅱ （CaMKⅡ） contributes to the analgesic effect of gabapentin on a chronic constriction injury （CCI） model. Methods Gabapentin （2%, 100 mg/kg） or saline （0.5 ml/100 g） was injected intraperitoneally 15 minutes prior to surgery and then every 12 hours from postoperative day 0-4 to all rats in control, sham and CCI groups. The analgesic effect of gabapentin was assessed by measuring mechanical allodynia and thermal hyperalgesia of rats. Expression and activation of CaMKⅡ were quantified by reverse-transcriptional polymerase chain reaction and Western blotting. Results The analgesic effect of gabapentin on mechanical allodynia and thermal hyperalgesia was significant in the CCI model, with maximal reduction reached on postoperative day 8. Gabapentin decreased the expression of the total CaMKⅡ and phosphorylated CaMKⅡ in CCI rats. Conclusion The analgesic effect of gabapentin on CCI rats may be related to the decreased expression and phosphorylation of CaMKⅡ in the spinal cord.