Details

Autor(en) / Beteiligte

• Chen, Xi
• Su, Chang
• Zhang, Zheng-Yun
• Zhang, Ming-Jun
• Gu, Wei-Qiong
• Li, Xiao-Ying
• Li, Hong-Wei
• Zhou, Guang-Wen

Titel

Hemo oxygenase-1 induction in vitro and in vivo can yield pancreas islet xenograft survival and improve islet function

Ist Teil von

• Chinese medical journal, 2011-10, Vol.124 (20), p.3378-3385

Ort / Verlag

China: Department of Surgery Shanghai First Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China%Department of Endocrine and Metabolism Shanghai First Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China%Ruijin Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China%Department of Surgery, Shanghai First Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China

Erscheinungsjahr

2011

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Background The induced expression of heme oxygenase-1 （HO-1） in donor islets improves allograft survival. Cobalt protoporphyrin （CoPP） could significantly enhance the expression of HO-1 mRNA and protein in rat islet safely. Our work was to study how to protect pancreatic islet xenograft by CoPP-induction. Methods Islet xenografts treated with CoPP-induction and CoPP＋ Zinc protoporphyrin （ZnPP） in vitro and in vivo were randomly transplanted into murine subrenal capsule; then the graft survival time was compared by blood glucose level and pathological examination and meanwhile the interferon ~ （IFN-y）, tumor necrosis factor a （TNF-a）, interleukin 10 （IL-10） and IL-113 level in serum and their mRNA and HO-1 mRNA and protein expression were examined. Results Islets with CoPP-induction under low- and high-glucose stimulation exhibited much higher insulin secretion compared with other three groups. CoPP-induction could increase higher expression of HO-1 （mRNA： 3.33- and 76.09- fold in vitro and in vivo; protein： 2.85- and 58.72-fold）. The normoglycemia time in induction groups （（14.63±1.19） and （16.88±1.64） days） was significantly longer. The pathological examination showed less lymphocyte infiltration in induction groups. The IL-10 level and its mRNA in induction groups were significantly higher. Conclusions The HO-1 induced by CoPP would significantly improve function, prolong normoglycemia time and reduce lymphocyte infiltration. Meanwhile CoPP-induction in vivo had more beneficial effects than in vitro. Its mechanism could be related to immune-modulation of IL-10.