Details

Autor(en) / Beteiligte

• Lin, Xing-guang
• Zhou, Qi
• Wang, Li
• Gao, Ying
• Zhang, Wei-na
• Luo, Zhen-long
• Chen, Bi-cheng
• Chen, Zhong-hua
• Chang, Sheng

Titel

Pregnancy estrogen drives the changes of T-lymphocyte subsets and cytokines and prolongs the survival of H-Y skin graft in murine model

Ist Teil von

• Chinese medical journal, 2010-09, Vol.123 (18), p.2593-2599

Ort / Verlag

China: Institute of Organ Transplantation, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Key Laboratory of Ministry of Health, and Key Laboratory of Ministry of Education, Wuhan, Hubei 430030, China%Department of Hepatobiliary Surgery, First Affiliated Hospital, Sun Yat-sen University, Guangzhou, Guangdong 510080, China%Wenzhou Key Laboratory of Surgery, Zhejiang Provincial Top Key Discipline in Surgery, First Affiliated Hospital of Wenzhou Medical College, Wenzhou, Zhejiang 325000, China

Erscheinungsjahr

2010

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• Background Estrogen as well as CD4＋Foxp3＋ regulatory T cells were shown to have a protective role not only in maintaining maternal-fetal tolerance but also against autoimmune diseases. We aimed to investigate whether the pregnancy levels of estrogen are enough to induce transplant tolerance as to maintain fetal-maternal tolerance. Methods We established H-Y skin graft transplantation in C57BL/6 ovariectomized mice that reconstituted with estrogen. Subsequently, consecutive daily estrogen injection was administrated. Tregs and the cytokines in the peripheral blood were detected by flow cytometry and ELISA pre- and post-transplant. Results The results indicated that pregnancy levels of estrogen could promote Tregs in secondary lymphoid organs and peripheral blood （P 〈0.05） but not thymus （P 〉0.05）. The estrogen-treated recipients accepted H-Y skin grafts for more than 35 days （median survival time （MST）： （44.0_＋1.2） days） compared with estrogen-untreated mice （MST： （23.0_＋1.6） days） （P 〈0.05）. It was also observed that estrogen up-regulated the expression of Foxp3, but did not affect CD3＋CD8＋ effector T-cells in non-transplant mice. While in the presence of H-Y antigens, the expression of Foxp3 was more significant and CD3＋CD8＋ effector T cells were decreased significantly （P 〈0.05）. Meanwhile, the up-regulated IL-10 and IL-4, and down-regulated IFN-v could be observed （P 〈0.05）. Conclusions Pregnancy levels of estrogen may promote the conversion of peripheral Tregs in secondary lymphoid organs, but show no effect on the natural Tregs production, differentiation and maturity in central lymphoid organs. Furthermore, pregnancy levels of estrogen could significantly prolong the survivals of H-Y skin grafts by the expansion of TreQs, suppression of CD3＋CD8＋ effector T-cells and immune shift towards Th2 cvtokines.