Details

Autor(en) / Beteiligte

• Chen, Shi-da
• Chen, Ya-bin
• Peng, You
• Xu, Jia
• Chen, Su-shan
• Zhang, Jun-long
• Li, Zheng-zhang
• Tan, Zhi

Titel

Role of PI3K/Akt signaling in the protective effect of magnesium sulfate against ischemia-perfusion injury of small intestine in rats

Ist Teil von

• Chinese medical journal, 2010-06, Vol.123 (11), p.1447-1452

Ort / Verlag

China: Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong 510080, China%Department of Physiology, Zhongshan School of Medicine, Sun Yat-sen University, Guangzhou, Guangdong 510080, China

Erscheinungsjahr

2010

Quelle

MEDLINE

Beschreibungen/Notizen

• Background The protective effects of magnesium sulfate against ischemia-reperfusion injury of the small intestine in Sprague-Dawley （SD） rats have been confirmed in our previous research. However, its exact mechanism is unclear. This study was to evaluate the role of PI3K/Akt signal pathway in the protective effect of magnesium sulfate against ischemia-reperfusion injury of the small intestine in SD rats. Methods Rat model of intestinal ischemia-reperfusion injury was used. The SD rats were divided into four groups randomly： sham operation group, ischemia-reperfusion group, magnesium sulfate group and magnesium sulfate plus LY294002 （an inhibitor of PI3K） group. The pathological changes of intestinal mucosa were examined; the activity of diamine oxidase （DAO） in plasma, the plasma contents of malondialdehyde （MDA）, and apoptosis rate of the intestinal mucosal cells were determined and compared. The expression of p-Akt was detected by Western blotting. Results There were more evident pathological changes of the intestinal mucosa （higher Chiu＇s score, P 〈0.05）, enhanced DAO activity （P 〈0.05）, elevated contents of MDA （P 〈0.05）, higher apoptosis rate （P 〈0.05）, and lower level of p-Akt （P 〈0.05） in the ischemia-reperfusion group compared with the sham operation group. There were less evident pathological changes of the intestinal mucosa （lower Chiu＇s score, P 〈0.05）, lower DAO activity （P 〈0.05）, lower contents of MDA （P 〈0.05）, and lower apoptosis rate （P 〈0.05）, but higher level of p-Akt （P 〈0.05） irL the magnesium sulfate group compared with the ischemia-reperfusion group. There were more evident pathological changes of the intes,inal mucosa （higher Chiu＇s score, P 〈0.05）, higher contents of MDA （P 〈0.05）, higher DAO activity （P 〈0.05） and higher apoptosis rate （P 〈0.05）, and lower level of p-Akt （P 〈0.05） in the magnesium sulfate plus LY294002 group compared with the magnesium sulfate group. Conclusions Activation of PI3K/Akt signal pathway results in the reduction of cell apoptosis, which likely accounts for the protective effect of magnesium sulfate against intestinal ischemia-reperfusion injury.