Details

Autor(en) / Beteiligte

• Tiancheng Wang Jing Wang Bin Geng Hongyu Guo Haili Shen Yayi Xia

Titel

Astroglial glutamate-glutamine cycle is involved in the modulation of inflammatory nociception in rats

Ist Teil von

• 中国神经再生研究（英文版）, 2011-12, Vol.6 (35), p.2746-2750

Ort / Verlag

Department of Neurology, Second Hospital of Lanzhou University, Lanzhou University, Lanzhou 730030, Gansu Province, China%Institute of Orthopedics, Second Hospital of Lanzhou University, Lanzhou University, Lanzhou 730030, Gansu Province, China%Department of Obstetrics and Gynecology, Second Hospital of Lanzhou University, Lanzhou University, Lanzhou 730030, Gansu Province, China%Department of Pain, Second Hospital of Lanzhou University, Lanzhou University, Lanzhou 730030, Gansu Province, China

Erscheinungsjahr

2011

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Our previous behavioral studies have indicated that the astroglial glutamate-giutamine cycle is involved in the process of formalin-induced spinal cord central sensitization, but there was little morphological evidence. In this study, double-labeling immunofluorescence techniques showed that after rats were intrathecally injected with PBS and plantarly injected with formalin, glial fibrillary acidic protein （GFAP） and glutamine synthesase （GS） expression were increased and GFAP/GS coexpression was changed to include layers III and IV. After intrathecal injection of methionine sulfoximine, a GS specific inhibitor, the formalin-induced change in expression and coexpression of GFAP and GS in spinal cord dorsal horns was inhibited. The morphology, distribution and quantity of astrocytes recovered to normal levels. An intrathecal glutamine injection reversed the inhibitory effect of methionine sulfoximine. Astrocytes showed significant activation and distribution extended to layers V and VI. The present study provides morphological evidence that the astroglial glutamateglutamine cycle is involved in the process of formalin-induced spinal cord central sensitization.