Details

Autor(en) / Beteiligte

• Xianjie Wen Shiyuan Xu Lingling Wang Hua Liang Chengxiang Yang Hanbing Wang Hongzhen Liu

Titel

T-type calcium channel expression in cultured human neuroblastoma cells

Ist Teil von

• 中国神经再生研究（英文版）, 2011-12, Vol.6 (31), p.2410-2413

Ort / Verlag

Department of Anesthesiology, Foshan Hospital of Sun Yat-sen University & First People's Hospital of Foshan, Foshan 528000, Guangdong Province,China%Department of Anesthesiology, Zhujiang Hospital of Southern Medical University, Guangzhou 510282, Guangdong Province, China%Department of Anesthesiology, Foshan Hospital of Sun Yat-sen University & First People's Hospital of Foshan, Foshan 528000, GuangdongProvince, China

Erscheinungsjahr

2011

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• Human neuroblastoma cells （SH-SY5Y） have similar structures and functions as neural cells and have been frequently used for cell culture studies of neural cell functions.Previous studies have revealed L-and N-type calcium channels in SH-SY5Y cells.However,the distribution of the low-voltage activated calcium channel （namely called T-type calcium channel,including Cav3.1,Cav3.2,and Cav3.3） in SH-SY5Y cells remains poorly understood.The present study detected mRNA and protein expres-sion of the T-type calcium channel （Cav3.1,Cav3.2,and Cav3.3） in cultured SH-SY5Y cells using real-time polymerase chain reaction （PCR） and western blot analysis.Results revealed mRNA and protein expression from all three T-type calcium channel subtypes in SH-SY5Y cells.Moreover,Cav3.1 was the predominant T-type calcium channel subtype in SH-SY5Y cells.