Agricultural sciences in China, 2009-12, Vol.8 (12), p.1419-1428
2009
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Details
- Autor(en) / Beteiligte
- Titel
- 1B Specific LMW-GS Primers Cloned a 1D Located Gene from Wheat Cultivar Xiaoyan 22
- Ist Teil von
- Agricultural sciences in China, 2009-12, Vol.8 (12), p.1419-1428
- Ort / Verlag
- Wheat Engineering Research Center of Shaanxi Province,Yangling 712100,PR.China
- Erscheinungsjahr
- 2009
- Quelle
- Alma/SFX Local Collection
- Beschreibungen/Notizen
- In the present study, one unique low-molecular-weight glutenin subunit （LMW-GS） gene. LMWXY22-2 （GenBank no. FJ028810）, was isolated from wheat cultivar Xiaoyan 22 （Triticum aestivum L.） by a pair of genomic specific PCR primers for 1B chromosome. Sequence analysis revealed that LMWXY22-2 was composed of 1 364 bp nucleotides, including a 317 bp promotion region and a 1 047 bp coding region which could be translated into a mature protein of 349 amino acids. In spite of a few minor mutations, the sequence of 5＇ untranslated region （UTR）, the coding region, the deduced N- and Cterminus comparisons indicated that LMWXY22-2 belonged to the reported subunits of LMW-m type and type lII group 5, respectively. Inner gene markers for 1D chromosome together with the phylogenetic analysis revealed that this gene was classified into Glu-D3, which was not in agreement with the I B locus-specific primers for LMW genes completely.