Details

Autor(en) / Beteiligte

• LIANG, Zhi-hong
• LI, Jun-xia
• HE, Yun-long
• GUAN, Shu
• WANG, Nin
• JI, Cheng
• NIU, Tian-gui

Titel

AFB1 Bio-Degradation by a New Strain - Stenotrophomonas. sp

Ist Teil von

• Agricultural sciences in China, 2008-12, Vol.7 (12), p.1433-1437

Ort / Verlag

College of Food Science & Nutritional Engineering,China Agricultural University,Beijing 100083,P.R.China%College of Animal Science and Technology,China Agricultural University,Beijing 100193,P.R.China

Erscheinungsjahr

2008

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• S6; The paper was to find the bacteria to degrade aflatoxin B 1 (AFB 1) and realize the application of biological degradation on AFB1. Using cumarin as the carbon source and energy on the first screening, then the ten strains which were first screened out were taken to degrade AFB 1 1 00 μg kg-1. Strain NMO-3 was screened out of ten strains, the degradation ratio of AFB1 reached 85.7%, which was more prominent than the others (P < 0.01). With the analysis of colony morphology, physiological and biochemistry experiments, and 16S rDNA gene sequence, the strain NMO-3 was finally identified as Stenotrophomonas sp. Using cumarin as the carbon source and energy could screen out the AFB1 degradation strains. Acute toxicity tests show that the viable number of NMO-3 lower than 3.12×1010 cfu mL-1 is safety. The crude enzyme was obtained by 65% ammonium sulfate fractionation, and it could degrade AFB1. It is the first report for the strain's detoxi-AFB1.