Details

Autor(en) / Beteiligte

• Liu, Lupeng
• Wang, Fengping
• Xu, Jun
• Xiao, Xiang

Titel

Molecular diversity of Thermococcales isolated from Guaymas Basin hydrothermal vents

Ist Teil von

• Acta oceanologica Sinica, 2013-06, Vol.32 (6), p.75-81

Ort / Verlag

Heidelberg: The Chinese Society of Oceanography

Erscheinungsjahr

2013

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Members of Thermococcales are organoheterotrophic hyperthermophilic anaerobes consisting of three genera, Thermococcus, Pyrococcus, and Palaeococcus, among which Thermococcus are the most frequently isolated. In this study, a variety of Thermococcales strains were isolated from the Guaymas Basin hydrother- mal vents under different temperatures and pressures. Based on their 16S rRNA gene sequences, all the s- trains isolated from 85℃ or 95℃ enrichment cultures at ambient pressure were classified as Thermococcus, while strains isolated from 108℃-30 MPa enrichment cultures belonged to Pyrococcus. The combination of high pressure and high temperature for enrichment was shown to be an efficient method for isolating Pyro- coccus strains. Through selecting and analyzing with multiple molecular phylogenetic markers, the isolated Thermococcales strains were found quite diversified including several putative novel species. This demon- strates a vast genetic reservoir of Thermoccoccales in the deep-sea hydrothermal vents, and a fast evolving of the Thermococcales species adapting to the fast changing environment.