Details

Autor(en) / Beteiligte

• DUAN Na WANG Xinyan ZHANG Chen ZHANG Yi HU Jun

Titel

Damage of DNA ends induced by mechanical force during AFM nano-manipulation

Ist Teil von

• 核技术（英文版）, 2013-02, Vol.24 (1), p.26-31

Ort / Verlag

University of Chinese Academy of Sciences, Beijing 100049, China%Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai 201800, China

Erscheinungsjahr

2013

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• An experimental and statistical study was carried out to explore the effects of mechanical forces on the ends of linear double-stranded DNA （dsDNA） fragments. Mechanical force was applied onto individual DNA molecules during atomic force microscope （AFM）-based picking-up manipulation. By comparing the PCR efficiency of two DNA fragments with primers either at ends or at the inner regions, it was found that the ends of DNA fragments were damaged during picking-up process