Details

Autor(en) / Beteiligte

• Sun, Tianxu
• Hou, Hongjia
• Lu, Jingcai
• Xu, Man
• Gu, Tiejun
• Wang, Dandan
• Dong, Yunliang
• Jiang, Chunlai
• Kong, Wei
• Wu, Yongge

Titel

Detection and comparison of structure and function of wild-type pneumolysin and its novel mutant PlyM2

Ist Teil von

• Chemical research in Chinese universities, 2015-08, Vol.31 (4), p.553-557

Ort / Verlag

Changchun: Jilin University and The Editorial Department of Chemical Research in Chinese Universities

Erscheinungsjahr

2015

Link zum Volltext

Quelle

SpringerLink (Online service)

Beschreibungen/Notizen

• Here is reported a novel pneumolysin(Ply) mutant(PlyM2) that addresses a long-standing problem for vaccine development in this field: detoxification of Ply in the premise of retaining antigenic integrity. Structure and function of wild-type Ply(PlyWT) and PlyM2 mutants were detected and compared. Their structures were not significantly different according to the analysis by thermal-dependent fluorescence spectroscopy and circular dichroism spectroscopy. PlyM2 was confirmed to have lost hemolytic activity and yet could induce neutralizing antibodies to prevent in vitro hemolysis by PlyWT and S. Pneumoniae . These results give support to PlyM2 to be a new protein antigen for inclusion in the development of an effective pneumococcal multiprotein vaccine.