Details

Autor(en) / Beteiligte

• ZHAO, Zhi Jing
• WANG, Bin
• YUAN, Jing
• LIANG, Hao Yu
• DONG, Si Guo
• ZENG, Ming

Titel

Examine the Correlation between Heat Shock Protein IbpA and Heat Tolerance in Cronobacter sakazakii

Ist Teil von

• Biomedical and environmental sciences, 2017-08, Vol.30 (8), p.606-610

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• We used a proteomic approach to identify IbpA in Cronobacter sakazokii （C. sakazaki）, which is related to heat tolerance in this strain. The abundance of IbpA in C. sakazakii strains strongly increased after heat shock. C sakazakii CMCC 45402 ibpA deletion mutants were successfully constructed. The C. sakazakii CMCC 45402 AibpA and wild-type strains could not be distinguished based on colony morphology on LB agar plates or biochemical assays. The growth of the C. sakazakii CMCC 45402 AibpA mutant in heat shock conditions was indistinguishable from that of the isogenic wild-type, but showed greater heat resistance than E. coil O157：H7 strain CMCC 44828. This study suggests that the absence of a single ibpA gene has no obvious effect on the phenotype or heat resistance of the strain C. sakazakii CMCC 45402.