Biomedical and environmental sciences, 2010-08, Vol.23 (4), p.251-258
2010
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- Autor(en) / Beteiligte
- Titel
- Proteome Analysis of Neisseria meningitidis Serogroup Strains C Associated with Outbreaks in China
- Ist Teil von
- Biomedical and environmental sciences, 2010-08, Vol.23 (4), p.251-258
- Ort / Verlag
- China: Elsevier B.V
- Erscheinungsjahr
- 2010
- Quelle
- EZB-FREE-00999 freely available EZB journals
- Beschreibungen/Notizen
- Objective During 2003-2005, an outbreak of meningitis due to Neisseria meningitidis serogroup C occurred in China. With the aim to find strain clues result in the final epidemics, the ancestral strain 053442, a clinical isolate, and a carrier strain 053426 with different gene type were analyzed. Methods Clinical strain 053442 and carrier strain 053426 were cultured on GC agar plates under the same condition. Two-dimensional electrophoresis was performed using the pH 3–10 nonlinear IPG strips of 24 cm length, and all the protein spots were identified by matrix-assisted laser desorption/ionization time of flight spectrometry. Results 502 and 380 protein spots were identified in 053426 and 053442 respectively, relating to 266 and 202 different genes covering a wide range of cellular functions. The express volume and number of proteins involved in energy metabolism, protein synthesis and amino acid biosynthesis in 053426 were higher than in 053442. Virulence factor Opa, Opc and a series of proteins involved in pilus assembly and retraction were identified in 053442, which appear to be of primary importance in colonization and invasion of human cells. Compared to 053442, virulence protein species were less in 053426, with lower express volumes too. No Opa and Opc were detected in 053426. Conclusion The different protein expression profiles of the clinical strain 053442 and carrier strain 053426 in the present study provide some clues of the different pathogenicity of the two strains, which may account for result in the final epidemics.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0895-3988eISSN: 2214-0190DOI: 10.1016/S0895-3988(10)60060-8Titel-ID: cdi_wanfang_journals_bes201004001
- Format
- –
- Schlagworte
- Bacterial Proteins - analysis, Bacterial Typing Techniques, China - epidemiology, Disease Outbreaks, Electrophoresis, Gel, Two-Dimensional, Humans, MALDI-TOF/TOF-MS, Meningitis, Meningococcal - cerebrospinal fluid, Meningitis, Meningococcal - epidemiology, Meningitis, Meningococcal - microbiology, Neisseria meningitidis, Neisseria meningitidis, Serogroup C - classification, Neisseria meningitidis, Serogroup C - isolation & purification, Proteome - analysis, Reference map, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization, Two-dimensional gel electrophoresis, 基质辅助激光解吸, 电离飞行时间质谱, 脑膜炎, 蛋白质合成, 蛋白质组, 血清组