Details

Autor(en) / Beteiligte

• Safi, C.
• Olivieri, G.
• Campos, R.P.
• Engelen-Smit, N.
• Mulder, W.J.
• van den Broek, L.A.M.
• Sijtsma, L.

Titel

Biorefinery of microalgal soluble proteins by sequential processing and membrane filtration

Ist Teil von

• Bioresource technology, 2017-02, Vol.225, p.151-158

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2017

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• Schematic representation of the overall process from cell disruption to diafiltration. [Display omitted] •A 100g.L−1 slurry of N. gaditana was submitted to protein biorefinery.•A biorefinery process was tested to disrupt the cell wall and filtrate the samples.•High-pressure homogenization was more efficient than enzymatic treatment.•The filtration process was more efficient after enzymatic treatment.•Increasing the cut off of the membrane does not improve the filtration process.•The mathematical model corresponded to the experimental data of filtration. A mild biorefinery process was investigated on the microalga Nannochloropsis gaditana, to obtain an enriched fraction of water soluble proteins free from chlorophyll. After harvesting, a 100g.L−1 solution of cells was first subjected to cell disruption by either high-pressure homogenization (HPH) or enzymatic treatment (ENZ). HPH resulted in a larger release of proteins (49%) in the aqueous phase compared to the Alcalase incubation (35%). In both cases, an ultrafiltration/diafiltration (UF/DF) was then performed on the supernatant obtained from cell disruption by testing different membrane cut-off (1000kDa, 500kDa and 300kDa). After optimising the process conditions, the combination of ENZ→UF/DF ended in a larger overall yield of water soluble proteins (24.8%) in the permeate compared to the combination of HPH→UF/DF (17.4%). A gel polarization model was implemented to assess the maximum achievable concentration factor during ultrafiltration and the mass transfer coefficient related to the theoretical permeation flux rate.