Details

Autor(en) / Beteiligte

• Coolen, Silvia
• Proietti, Silvia
• Hickman, Richard
• Davila Olivas, Nelson H.
• Huang, Ping‐Ping
• Van Verk, Marcel C.
• Van Pelt, Johan A.
• Wittenberg, Alexander H.J.
• De Vos, Martin
• Prins, Marcel
• Van Loon, Joop J.A.
• Aarts, Mark G.M.
• Dicke, Marcel
• Pieterse, Corné M.J.
• Van Wees, Saskia C.M.

Titel

Transcriptome dynamics of Arabidopsis during sequential biotic and abiotic stresses

Ist Teil von

• The Plant journal : for cell and molecular biology, 2016-05, Vol.86 (3), p.249-267

Ort / Verlag

England: Blackwell Publishing Ltd

Erscheinungsjahr

2016

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• Summary In nature, plants have to cope with a wide range of stress conditions that often occur simultaneously or in sequence. To investigate how plants cope with multi‐stress conditions, we analyzed the dynamics of whole‐transcriptome profiles of Arabidopsis thaliana exposed to six sequential double stresses inflicted by combinations of: (i) infection by the necrotrophic fungus Botrytis cinerea, (ii) herbivory by chewing larvae of Pieris rapae, and (iii) drought stress. Each of these stresses induced specific expression profiles over time, in which one‐third of all differentially expressed genes was shared by at least two single stresses. Of these, 394 genes were differentially expressed during all three stress conditions, albeit often in opposite directions. When two stresses were applied in sequence, plants displayed transcriptome profiles that were very similar to the second stress, irrespective of the nature of the first stress. Nevertheless, significant first‐stress signatures could be identified in the sequential stress profiles. Bioinformatic analysis of the dynamics of co‐expressed gene clusters highlighted specific clusters and biological processes of which the timing of activation or repression was altered by a prior stress. The first‐stress signatures in second stress transcriptional profiles were remarkably often related to responses to phytohormones, strengthening the notion that hormones are global modulators of interactions between different types of stress. Because prior stresses can affect the level of tolerance against a subsequent stress (e.g. prior herbivory strongly affected resistance to B. cinerea), the first‐stress signatures can provide important leads for the identification of molecular players that are decisive in the interactions between stress response pathways. Significance Statement Plants have to cope simultaneously with diverse and often interacting stresses. Here we analyzed the dynamics of whole genome transcriptome profiles of Arabidopsis plants in response to six combinations of sequential biotic and abiotic stresses. We found that the transcriptome profile of sequentially stressed plants is largely determined by the last stress encountered. First‐stress signatures in the second stress transcriptome provide leads to identify molecular players with decisive roles in positive or negative interactions between stress pathways.