Journal of cellular and molecular medicine, 2013-11, Vol.17 (11), p.1444-1457
2013
Details
- Autor(en) / Beteiligte
- Titel
- Calcium ions and osteoclastogenesis initiate the induction of bone formation by coral‐derived macroporous constructs
- Ist Teil von
- Journal of cellular and molecular medicine, 2013-11, Vol.17 (11), p.1444-1457
- Ort / Verlag
- England: John Wiley & Sons, Inc
- Erscheinungsjahr
- 2013
- Link zum Volltext
- Quelle
- Wiley Online Library
- Beschreibungen/Notizen
- Coral‐derived calcium carbonate/hydroxyapatite macroporous constructs of the genus Goniopora with limited hydrothermal conversion to hydroxyapatite (7% HA/CC) initiate the induction of bone formation. Which are the molecular signals that initiate pattern formation and the induction of bone formation? To evaluate the role of released calcium ions and osteoclastogenesis, 7% HA/CC was pre‐loaded with either 500 μg of the calcium channel blocker, verapamil hydrochloride, or 240 μg of the osteoclast inhibitor, biphosphonate zoledronate, and implanted in the rectus abdominis muscle of six adult Chacma baboons Papio ursinus. Generated tissues on days 15, 60 and 90 were analysed by histomorphometry and qRT‐PCR. On day 15, up‐regulation of type IV collagen characterized all the implanted constructs correlating with vascular invasion. Zoledronate‐treated specimens showed an important delay in tissue patterning and morphogenesis with limited bone formation. Osteoclastic inhibition yielded minimal, if any, bone formation by induction. 7% HA/CC pre‐loaded with the Ca++ channel blocker verapamil hydrochloride strongly inhibited the induction of bone formation. Down‐regulation of bone morphogenetic protein‐2 (BMP‐2) together with up‐regulation of Noggin genes correlated with limited bone formation in 7% HA/CC pre‐loaded with either verapamil or zoledronate, indicating that the induction of bone formation by coral‐derived macroporous constructs is via the BMPs pathway. The spontaneous induction of bone formation is initiated by a local peak of Ca++ activating stem cell differentiation and the induction of bone formation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1582-1838eISSN: 1582-4934DOI: 10.1111/jcmm.12125Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_4117557
- Format
- –
- Schlagworte
- Angiogenesis, Animals, Anthozoa - chemistry, Baboons, Biology, Biomedical materials, Bone Density Conservation Agents - pharmacology, Bone growth, Bone histomorphometry, Bone Morphogenetic Protein 2 - metabolism, Bone morphogenetic proteins, Bone Regeneration, Bone Substitutes - chemistry, Ca+, Calcium, Calcium - physiology, Calcium carbonate, Calcium Carbonate - chemistry, calcium channel blocker verapamil hydrochloride, Calcium channel blockers, Calcium Channel Blockers - pharmacology, Carrier Proteins - metabolism, Cell differentiation, Collagen, Collagen (type IV), Collagen Type IV - metabolism, Correlation analysis, Diphosphonates - pharmacology, Durapatite - chemistry, functionalized nano‐patterned topographies, Gene expression, Gene regulation, Geometry, Goniopora, Hydroxyapatite, Ions, Language, Molecules, Monkeys & apes, Morphogenesis, Morphology, Muscles, Noggin protein, Original, osteoclast inhibitor biphosphonate zoledronate, Osteoclastogenesis, Osteoclasts, Osteoclasts - drug effects, Osteoclasts - physiology, Osteogenesis, Papio, Papio ursinus, Pattern formation, Polymerase chain reaction, Porosity, Prostheses and Implants, qRT‐PCR, Stem cells, Tissue engineering, Transplants & implants, Verapamil, Verapamil - pharmacology, Zoledronic acid