Details

Autor(en) / Beteiligte

• Merhej, Vicky
• Adekambi, Toidi
• Pagnier, Isabelle
• Raoult, Didier
• Drancourt, Michel

Titel

Yersinia massiliensis sp. nov., isolated from fresh water

Ist Teil von

• International journal of systematic and evolutionary microbiology, 2008-04, Vol.58 (4), p.779-784

Ort / Verlag

Reading: Soc General Microbiol

Erscheinungsjahr

2008

Link zum Volltext

Quelle

EZB Electronic Journals Library

Beschreibungen/Notizen

• AP-HM Timone, Fédération de Microbiologie Clinique and Unité des Rickettsies CNRS UMR 6020, IFR 48, Faculté de Médecine, Université de la Méditerranée, Marseille, France Correspondence Michel Drancourt Michel.Drancourt{at}medecine.univ-mrs.fr Two bacterial organisms, 50640 T and 823, were isolated from fresh water in Marseilles, France, and were further identified as members of the genus Yersinia on the basis of their phenotypic characteristics and 16S rRNA gene sequencing. Their unique phenotypic profile differed from that of closely related species of Yersinia bercovieri and Yersinia mollaretii by exhibiting positive indole and inositol tests, and from that of Yersinia frederiksenii by lacking the ability to ferment L -rhamnose. A polyphasic approach, including almost complete 16S rRNA gene sequencing (1461 bp) and partial sequencing of hsp60 (683 bp), gyrB (662 bp), sodA (624 bp) and rpoB (1049 bp) showed that isolates 50640 T and 823 exhibited 98.5, 93.5, 90.4, 92.4 and 96.6 % similarity with Y. mollaretii , 98.7, 93.0, 90.1, 89.1 and 96.2 % with Y. bercovieri , and 98.4, 93.2, 89.8, 88.9 and 95.2 % with Y. frederiksenii , respectively. Both isolates exhibited an identical 16S rRNA gene sequence and differed by one to five point mutations in housekeeping gene sequences. Phylogenetic reconstructions based on the combination of these four housekeeping genes indicated that the two isolates formed a unique branch supported by a bootstrap value of 93 %. Their unique phenotypic traits, 16S rRNA gene sequence, together with housekeeping gene sequences exhibiting <97 % similarity with closely related species, and phylogenetic analyses suggested that the two isolates represent a so far undescribed Yersinia species. The name Yersinia massiliensis sp. nov. is proposed for this new taxon (type strain 50640 T =CIP 109351 T =CCUG 53443 T ; isolate 823=CIP 109352=CCUG 53444). The GenBank/EMBL/DDBJ accession numbers for the gene sequences of isolates 50640 T and 823 are EF179119 and EF179120 (16S rRNA gene), EF173172 and EF173177 ( hsp60 ), EF175588 and EF175591 ( gyrB ), EF175618 and EF175621 ( sodA ), and EF175599 and EF175600 ( rpoB ), respectively. Phylogenetic trees based on rpoB , hsp60 , gyrB and sodA gene sequences of isolates 50640 T and 823 and 11 Yersinia species are available with the online version of this paper.