The FASEB journal, 2012-10, Vol.26 (10), p.4057-4067
2012
Details
- Autor(en) / Beteiligte
- Titel
- Aurora B is regulated by acetylation/deacetylation during mitosis in prostate cancer cells
- Ist Teil von
- The FASEB journal, 2012-10, Vol.26 (10), p.4057-4067
- Ort / Verlag
- United States: The Federation of American Societies for Experimental Biology
- Erscheinungsjahr
- 2012
- Link zum Volltext
- Quelle
- Wiley Online Library Journals Frontfile Complete
- Beschreibungen/Notizen
- Protein acetylation has been implicated in playing an important role during mitotic progression. Aurora B kinase is known to play a critical role in mitosis. However, whether Aurora B is regulated by acetylation is not known. Using IP with an anti‐acetyl lysine antibody, we identified Aurora B as an acetylated protein in PC3 prostate cancer cells. Knockdown of HDAC3 or inhibiting HDAC3 deacetylase activity led to a significant increase (P<0.01 and P<0.05, respectively) in Aurora B acetylation as compared to siLuc or vehicle‐treated controls. Increased Aurora B acetylation is correlated with a 30% reduction in Aurora B kinase activity in vitro and resulted in significant defects in Aurora B‐dependent mitotic processes, including kinetochore‐microtubule attachment and chromosome congression. Furthermore, Aurora B transiently interacts with HDAC3 at the kinetochore‐microtubule interface of congressing chromosomes during prometaphase. This window of interaction corresponded with a transient but significant reduction (P=0.02) in Aurora B acetylation during early mitosis. Together, these results indicate that Aurora B is more active in its deacetylated state and further suggest a new mechanism by which dynamic acetylation/deacetylation acts as a rheostat to fine‐tune Aurora B activity during mitotic progression.—Fadri‐Moskwik, M., Weiderhold, K. N., Deeraksa, A., Chuang, C., Pan, J., Lin, S.‐H., Yu‐Lee, L.‐Y. Aurora B is regulated by acetylation/deacetylation during mitosis in prostate cancer cells. FASEB J. 26, 4057–4067 (2012). www.fasebj.org
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0892-6638eISSN: 1530-6860DOI: 10.1096/fj.12-206656Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_3448774
- Format
- –
- Schlagworte
- Acetylation, Aurora Kinase B, Aurora Kinases, Cell Line, Tumor, HDAC3, Histone Deacetylases - metabolism, Humans, Immunoblotting, Kinetochores - metabolism, kinetochore‐microtubule attachment, Male, Microscopy, Fluorescence, Microtubules - metabolism, Mitosis - genetics, Mitosis - physiology, mitotic spindle, post‐translational modification, Prostatic Neoplasms - enzymology, Prostatic Neoplasms - metabolism, Protein Processing, Post-Translational, Protein Serine-Threonine Kinases - metabolism, Research Communications