Scientific reports, 2018-02, Vol.8 (1), p.2445-16, Article 2445
2018
Details
- Autor(en) / Beteiligte
- Titel
- Silver nanoparticles have lethal and sublethal adverse effects on development and longevity by inducing ROS-mediated stress responses
- Ist Teil von
- Scientific reports, 2018-02, Vol.8 (1), p.2445-16, Article 2445
- Ort / Verlag
- England: Nature Publishing Group
- Erscheinungsjahr
- 2018
- Link zum Volltext
- Quelle
- Elektronische Zeitschriftenbibliothek (Open access)
- Beschreibungen/Notizen
- Silver nanoparticles (AgNPs) are widely used in the household, medical and industrial sectors due to their effective bactericidal activities and unique plasmonic properties. Despite the promising advantages, safety concerns have been raised over the usage of AgNPs because they pose potential hazards. However, the mechanistic basis behind AgNPs toxicity, particularly the sublethal effects at the organismal level, has remained unclear. In this study, we used a powerful in vivo platform Drosophila melanogaster to explore a wide spectrum of adverse effects exerted by dietary AgNPs at the organismal, cellular and molecular levels. Lethal doses of dietary AgNPs caused developmental delays and profound lethality in developing animals and young adults. In contrast, exposure to sublethal doses, while not deadly to developing animals, shortened the adult lifespan and compromised their tolerance to oxidative stress. Importantly, AgNPs mechanistically resulted in tissue-wide accumulation of reactive oxygen species (ROS) and activated the Nrf2-dependent antioxidant pathway, as demonstrated by an Nrf2 activity reporter in vivo. Finally, dietary AgNPs caused a variety of ROS-mediated stress responses, including apoptosis, DNA damage, and autophagy. Altogether, our study suggests that lethal and sublethal doses of AgNPs, have acute and chronic effects, respectively, on development and longevity by inducing ROS-mediated stress responses.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 2045-2322eISSN: 2045-2322DOI: 10.1038/s41598-018-20728-zTitel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5799281
- Format
- –
- Schlagworte
- Animals, Antioxidants, Apoptosis, Apoptosis - drug effects, Autophagy, Autophagy - drug effects, Chronic effects, DNA Damage, Dose-Response Relationship, Drug, Drosophila melanogaster - drug effects, Drosophila melanogaster - genetics, Drosophila melanogaster - growth & development, Drosophila melanogaster - metabolism, Drosophila Proteins - genetics, Drosophila Proteins - metabolism, Drug dosages, Gene Expression Regulation, Developmental - drug effects, Insects, Larva - drug effects, Larva - genetics, Larva - growth & development, Larva - metabolism, Lethality, Life span, Longevity, Longevity - drug effects, Male, Metal Nanoparticles - chemistry, Metal Nanoparticles - toxicity, Nanoparticles, Nanotechnology, NF-E2-Related Factor 2 - genetics, NF-E2-Related Factor 2 - metabolism, Oxidative Stress, Phagocytosis, Pupa - drug effects, Pupa - genetics, Pupa - growth & development, Pupa - metabolism, Reactive oxygen species, Reactive Oxygen Species - agonists, Reactive Oxygen Species - metabolism, Side effects, Silver, Silver - chemistry, Silver - toxicity, Survival Rate, Toxicity, Toxicity Tests, Acute, Toxicity Tests, Chronic