British journal of nutrition, 2018-08, Vol.120 (4), p.385-392
2018
Details
- Autor(en) / Beteiligte
- Titel
- Effects of SCFA on the DNA methylation pattern of adiponectin and resistin in high-fat-diet-induced obese male mice
- Ist Teil von
- British journal of nutrition, 2018-08, Vol.120 (4), p.385-392
- Ort / Verlag
- England: Cambridge University Press
- Erscheinungsjahr
- 2018
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- Specific adipokines, such as adiponectin and resistin, are secreted from adipose tissue and are associated with the development of obesity. Supplementation of dietary SCFA can prevent and reverse high-fat-diet (HFD)-induced obesity. However, it is not clear whether SCFA ameliorate abnormal expression of adiponectin and resistin in the obese state. The aim of this study was to investigate the effects of SCFA on adiponectin and resistin's expressions in diet-induced obese mice, as well as the potential mechanisms associated with DNA methylation. C57BL/6J male mice were fed for 16 weeks with five types of HFD (34·9 % fat by wt., 60 % kJ) - a control HFD and four HFD with acetate (HFD-A), propionate (HFD-P), butyrate (HFD-B) and their admixture (HFD-SCFA). Meanwhile, a low-fat diet (4·3 % fat by wt., 10 % kJ) was used as the control group. The reduced mRNA levels of adiponectin and resistin in the adipose tissue of the HFD-fed mice were significantly reversed by dietary supplementation of acetate, propionate, butyrate or their admixture to the HFD. Moreover, the expressional changes of adiponectin and resistin induced by SCFA were associated with alterations in DNA methylation at their promoters, which was mediated by reducing the expressions of enzyme-catalysed DNA methyltransferase (DNMT1, 3a, 3b) and the methyl-CpG-binding domain protein 2 (MBD2) and suppressing the binding of these enzymes to the promoters of adiponectin and resistin. Our results indicate that SCFA may correct aberrant expressions of adiponectin and resistin in obesity by epigenetic regulation.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0007-1145eISSN: 1475-2662DOI: 10.1017/s0007114518001526Titel-ID: cdi_proquest_miscellaneous_2057865407
- Format
- –
- Schlagworte
- Acetic acid, Adipokines - metabolism, Adiponectin, Adiponectin - metabolism, Adipose Tissue, Admixtures, Animal tissues, Animals, Binding, Binding Sites, Body Weight, CpG Islands, Deoxyribonucleic acid, Diet, Diet, High-Fat, Dietary Fats, Dietary supplements, DNA, DNA (Cytosine-5-)-Methyltransferase 1 - genetics, DNA (Cytosine-5-)-Methyltransferase 1 - metabolism, DNA Methylation, DNA methyltransferase, DNA-Binding Proteins - genetics, DNMT1 protein, Epigenesis, Genetic, Epigenetics, Fatty acids, Fatty Acids, Volatile - metabolism, Gene expression, High fat diet, Insulin resistance, Laboratory animals, Low fat diet, Male, Metabolism, Mice, Mice, Inbred C57BL, Mice, Obese, Microbiota, mRNA, Nutrient deficiency, Nutrition research, Obesity, Obesity - complications, Obesity - metabolism, Propionic acid, Proteins, Resistin - metabolism, RNA, Messenger - metabolism, Rodents, Sodium, Supplementation, Weight control