Cell, 2019-12, Vol.179 (7), p.1636-1646.e15
- Setliff, Ian
- Shiakolas, Andrea R.
- Pilewski, Kelsey A.
- Murji, Amyn A.
- Mapengo, Rutendo E.
- Janowska, Katarzyna
- Richardson, Simone
- Oosthuysen, Charissa
- Raju, Nagarajan
- Ronsard, Larance
- Kanekiyo, Masaru
- Qin, Juliana S.
- Kramer, Kevin J.
- Greenplate, Allison R.
- McDonnell, Wyatt J.
- Graham, Barney S.
- Connors, Mark
- Lingwood, Daniel
- Acharya, Priyamvada
- Morris, Lynn
- Georgiev, Ivelin S.
2019
Details
- Autor(en) / Beteiligte
- Setliff, Ian
- Shiakolas, Andrea R.
- Pilewski, Kelsey A.
- Murji, Amyn A.
- Mapengo, Rutendo E.
- Janowska, Katarzyna
- Richardson, Simone
- Oosthuysen, Charissa
- Raju, Nagarajan
- Ronsard, Larance
- Kanekiyo, Masaru
- Qin, Juliana S.
- Kramer, Kevin J.
- Greenplate, Allison R.
- McDonnell, Wyatt J.
- Graham, Barney S.
- Connors, Mark
- Lingwood, Daniel
- Acharya, Priyamvada
- Morris, Lynn
- Georgiev, Ivelin S.
- Titel
- High-Throughput Mapping of B Cell Receptor Sequences to Antigen Specificity
- Ist Teil von
- Cell, 2019-12, Vol.179 (7), p.1636-1646.e15
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2019
- Link zum Volltext
- Quelle
- Elsevier ScienceDirect Journals Complete
- Beschreibungen/Notizen
- B cell receptor (BCR) sequencing is a powerful tool for interrogating immune responses to infection and vaccination, but it provides limited information about the antigen specificity of the sequenced BCRs. Here, we present LIBRA-seq (linking B cell receptor to antigen specificity through sequencing), a technology for high-throughput mapping of paired heavy- and light-chain BCR sequences to their cognate antigen specificities. B cells are mixed with a panel of DNA-barcoded antigens so that both the antigen barcode(s) and BCR sequence are recovered via single-cell next-generation sequencing. Using LIBRA-seq, we mapped the antigen specificity of thousands of B cells from two HIV-infected subjects. The predicted specificities were confirmed for a number of HIV- and influenza-specific antibodies, including known and novel broadly neutralizing antibodies. LIBRA-seq will be an integral tool for antibody discovery and vaccine development efforts against a wide range of antigen targets. [Display omitted] •LIBRA-seq: high-throughput mapping of BCR sequence to antigen specificity•Identified HIV- and influenza-specific B cells in two HIV-infected subjects•Predicted antigen reactivity for thousands of single B cells•Identified a previously unknown broadly neutralizing HIV antibody LIBRA-seq enables high-throughput mapping of B cell receptor sequence to antigen specificity at the single-cell level.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0092-8674eISSN: 1097-4172DOI: 10.1016/j.cell.2019.11.003Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_7158953
- Format
- –
- Schlagworte
- antibodies, Antibodies, Neutralizing - chemistry, Antibodies, Neutralizing - immunology, antibody discovery, antibody repertoire, Antigens - chemistry, Antigens - immunology, B cells, broadly neutralizing antibody, Cells, Cultured, Epitope Mapping - methods, Epitopes - chemistry, Epitopes - immunology, HEK293 Cells, High-Throughput Nucleotide Sequencing - methods, High-Throughput Screening Assays - methods, HIV, HIV Antibodies - chemistry, HIV Antibodies - immunology, Humans, influenza, Receptors, Antigen, B-Cell - chemistry, Receptors, Antigen, B-Cell - immunology, Sequence Analysis, DNA - methods, single cell immunology, Single-Cell Analysis - methods, systems immunology, THP-1 Cells