Biochimica et biophysica acta, 2015-10, Vol.1854 (10), p.1667-1675
2015
Details
- Autor(en) / Beteiligte
- Titel
- Neurochondrin is an atypical RIIα-specific A-kinase anchoring protein
- Ist Teil von
- Biochimica et biophysica acta, 2015-10, Vol.1854 (10), p.1667-1675
- Ort / Verlag
- Netherlands: Elsevier B.V
- Erscheinungsjahr
- 2015
- Link zum Volltext
- Quelle
- Elsevier ScienceDirect Journals Complete
- Beschreibungen/Notizen
- Protein kinase activity is regulated not only by direct strategies affecting activity but also by spatial and temporal regulatory mechanisms. Kinase signaling pathways are coordinated by scaffolding proteins that orchestrate the assembly of multi-protein complexes. One family of such scaffolding proteins are the A-kinase anchoring proteins (AKAPs). AKAPs share the commonality of binding cAMP-dependent protein kinase (PKA). In addition, they bind further signaling proteins and kinase substrates and tether such multi-protein complexes to subcellular locations. The A-kinase binding (AKB) domain of AKAPs typically contains a conserved helical motif that interacts directly with the dimerization/docking (D/D) domain of the regulatory subunits of PKA. Based on a pull-down proteomics approach, we identified neurochondrin (neurite-outgrowth promoting protein) as a previously unidentified AKAP. Here, we show that neurochondrin interacts directly with PKA through a novel mechanism that involves two distinct binding regions. In addition, we demonstrate that neurochondrin has strong isoform selectivity towards the RIIα subunit of PKA with nanomolar affinity. This article is part of a Special Issue entitled: Inhibitors of Protein Kinases •Neurochondrin is a new A-kinase anchoring protein.•Neurochondrin has a strong isoform selectivity towards the RIIα subunit.•Neurochondrin interacts with the RIIα isoform with nanomolar affinity.•Neurochondrin interacts directly with PKA through a novel mechanism.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1570-9639, 0006-3002eISSN: 1878-1454, 1878-2434DOI: 10.1016/j.bbapap.2015.04.018Titel-ID: cdi_crossref_primary_10_1016_j_bbapap_2015_04_018
- Format
- –
- Schlagworte
- A Kinase Anchor Proteins - chemistry, A Kinase Anchor Proteins - metabolism, A-kinase anchoring proteins, Amino Acid Sequence, Binding Sites, Cyclic AMP - metabolism, Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit - chemistry, Cyclic AMP-Dependent Protein Kinase RIIalpha Subunit - metabolism, Cyclic AMP-Dependent Protein Kinases - chemistry, Cyclic AMP-Dependent Protein Kinases - metabolism, Humans, Multiprotein Complexes, Nerve Tissue Proteins - chemistry, Nerve Tissue Proteins - metabolism, Neurochondrin, Protein Binding, Protein kinase A, RII-specific, Signal Transduction