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Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association, 2017, Vol.20 (1), p.126-135
Monitoring the HER2 copy number status in circulating tumor DNA by droplet digital PCR in patients with gastric cancer
Ist Teil von
Gastric cancer : official journal of the International Gastric Cancer Association and the Japanese Gastric Cancer Association, 2017, Vol.20 (1), p.126-135
Ort / Verlag
Tokyo: Springer Japan
Erscheinungsjahr
2017
Link zum Volltext
Quelle
SpringerLink (Online service)
Beschreibungen/Notizen
Background
We previously demonstrated the potential of circulating tumor DNA (ctDNA) for the amplification of detecting
HER2
in patients with gastric cancer (GC). In the present study, we focused on the clinical courses of patients who developed recurrence with GC, and investigated the potential clinical utility of the ddPCR-based
HER2
copy number (CN) as a marker for the temporal and/or spatial heterogeneities of GC during treatment progress.
Method
We enrolled 30 healthy volunteers and 60 patients with GC who underwent surgery, including 17 patients who developed recurrence. Using ribonuclease P RNA component H1 (
RPPH1
) as a reference gene, plasma
HER2
to
RPPH1
ratios (the
HER2
ratio) were determined using ddPCR.
Results
The preoperative plasma
HER2
ratio correlated with the tumor HER2 status (
p
< 0.001), and sensitivity and specificity were 0.733 and 0.933, respectively. Analyses of plasma samples during the postoperative follow-up periods revealed that high plasma
HER2
ratios were detected at the time of recurrence in 7 of 13 cases, which were diagnosed as being HER2 negative at the time of surgery. These results were supported by continuously increasing
HER2
ratios thereafter with the progression of recurrent cancer.
Conclusion
The plasma
HER2
ratio determined by ddPCR is a repeatable and noninvasive approach for real-time evaluations of the HER2 status to monitor the effects of treatments for patients with HER2-positive GC and enable treatment options for patients with HER2-negative GC but positive conversion of the HER2 status after recurrence.