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Key message
Overexpression of
JcGAST1
promotes plant growth but inhibits pistil development. The pyrimidine box and CGTCA motif of the
JcGAST1
promoter were responsible for the GA and MeJA responses.
Members of the gibberellic acid-stimulated
Arabidopsis
(GASA) gene family play roles in plant growth and development, particularly in flower induction and seed development. However, there is still relatively limited knowledge of GASA genes in
Jatropha curcas
. Herein, we identified a GASA family gene from
Jatropha curcas
, namely,
JcGAST1
, which encodes a protein containing a conserved GASA domain. Sequence alignment showed that the JcGAST1 protein shares 76% sequence identity and 80% sequence similarity with SlGAST1.
JcGAST1
had higher expression and protein levels in the female flowers than in the male flowers. Overexpression of
JcGAST1
in tobacco promotes plant growth but inhibits pistil development.
JcGAST1
expression was upregulated by GA and downregulated by MeJA. Promoter analysis indicated that the pyrimidine box and CGTCA motif were the GA- and MeJA-responsive elements of the
JcGAST1
promoter. Using a Y1H screen, six transcription factors were found to interact with the pyrimidine box, and three transcription factors were found to interact with the CGTCA motif. Overall, the results of this study improve our understanding of the
JcGAST1
gene and provide useful information for further studies.