Stem cells (Dayton, Ohio), 2016-11, Vol.34 (11), p.2772-2783
2016
Details
- Autor(en) / Beteiligte
- Titel
- Transcription Factor GLIS3: A New and Critical Regulator of Postnatal Stages of Mouse Spermatogenesis
- Ist Teil von
- Stem cells (Dayton, Ohio), 2016-11, Vol.34 (11), p.2772-2783
- Ort / Verlag
- United States: Oxford University Press
- Erscheinungsjahr
- 2016
- Link zum Volltext
- Quelle
- Free E-Journal (出版社公開部分のみ）
- Beschreibungen/Notizen
- In this study, we identify a novel and essential role for the Krüppel‐like zinc finger transcription factor GLI‐similar 3 (GLIS3) in the regulation of postnatal spermatogenesis. We show that GLIS3 is expressed in gonocytes, spermatogonial stem cells (SSCs) and spermatogonial progenitors (SPCs), but not in differentiated spermatogonia and later stages of spermatogenesis or in somatic cells. Spermatogenesis is greatly impaired in GLIS3 knockout mice. Loss of GLIS3 function causes a moderate reduction in the number of gonocytes, but greatly affects the generation of SSCs/SPCs, and as a consequence the development of spermatocytes. Gene expression profiling demonstrated that the expression of genes associated with undifferentiated spermatogonia was dramatically decreased in GLIS3‐deficient mice and that the cytoplasmic‐to‐nuclear translocation of FOXO1, which marks the gonocyte‐to‐SSC transition and is necessary for SSC self‐renewal, is inhibited. These observations suggest that GLIS3 promotes the gonocyte‐to‐SSC transition and is a critical regulator of the dynamics of early postnatal spermatogenesis. Stem Cells 2016;34:2772–2783
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1066-5099eISSN: 1549-4918DOI: 10.1002/stem.2449Titel-ID: cdi_pubmedcentral_primary_oai_pubmedcentral_nih_gov_5584072
- Format
- –
- Schlagworte
- Animals, Cell Differentiation, Cell self-renewal, DNA-Binding Proteins, Forkhead Box Protein O1 - genetics, Forkhead Box Protein O1 - metabolism, FOXO1 protein, Gene expression, Gene Expression Profiling, Gene Expression Regulation, Developmental, GLIS3, Kruppel protein, Male, Mice, Mice, Inbred C57BL, Mice, Knockout, Nuclear transport, Protein Transport, Repressor Proteins - deficiency, Repressor Proteins - genetics, Rodents, Scleroderma, Somatic cells, Spermatocytes, Spermatocytes - cytology, Spermatocytes - metabolism, Spermatogenesis, Spermatogenesis - genetics, Spermatogonia, Spermatogonia - cytology, Spermatogonia - metabolism, Spermatogonial stem cell, Stem cell transplantation, Stem cells, Stem Cells - cytology, Stem Cells - metabolism, Testis - cytology, Testis - metabolism, Trans-Activators - deficiency, Trans-Activators - genetics, Transcription factors, Translocation, Zinc, Zinc finger proteins