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  • BibTeX
RNAi Targeting Putative Genes in Phosphatidylcholine Turnover Results in Significant Change in Fatty Acid Composition in Crambe abyssinica Seed Oil
Lipids, 2015-04, Vol.50 (4), p.407-416
2015

Details

Autor(en) / Beteiligte
Titel
RNAi Targeting Putative Genes in Phosphatidylcholine Turnover Results in Significant Change in Fatty Acid Composition in Crambe abyssinica Seed Oil
Ist Teil von
  • Lipids, 2015-04, Vol.50 (4), p.407-416
Ort / Verlag
Berlin/Heidelberg: Springer Berlin Heidelberg
Erscheinungsjahr
2015
Link zum Volltext
Quelle
Wiley Online Library Journals Frontfile Complete
Beschreibungen/Notizen
  • The aim of this study was to evaluate the importance of three enzymes, LPCAT, PDCT and PDAT, involved in acyl turnover in phosphatidylcholine in order to explore the possibility of further increasing erucic acid (22:1) content in Crambe seed oil. The complete coding sequences of LPCAT1 - 1 and LPCAT1 - 2 encoding lysophosphatidylcholine acyltransferase (LPCAT), PDCT1 and PDCT2 encoding phosphatidylcholine:diacylglycerol cholinephosphotransferase (PDCT), and PDAT encoding phospholipid:diacylglycerol acyltransferase (PDAT) were cloned from developing Crambe seeds. The alignment of deduced amino acid sequences displayed a high similarity to the Arabidopsis homologs. Transgenic lines expressing RNA interference (RNAi) targeting either single or double genes showed significant changes in the fatty acid composition of seed oil. An increase in oleic acid (18:1) was observed, to varying degrees, in all of the transgenic lines, and a cumulative effect of increased 18:1 was shown in the LPCAT - PDCT double-gene RNAi. However, LPCAT single-gene RNAi led to a decrease in 22:1 accumulation, while PDCT or PDAT single-gene RNAi had no obvious effect on the level of 22:1. In agreement with the abovementioned oil phenotypes, the transcript levels of the target genes in these transgenic lines were generally reduced compared to wild-type levels. In this paper, we discuss the potential to further increase the 22:1 content in Crambe seed oil through downregulation of these genes in combination with fatty acid elongase and desaturases.
Sprache
Englisch
Identifikatoren
ISSN: 0024-4201, 1558-9307
eISSN: 1558-9307
DOI: 10.1007/s11745-015-4004-1
Titel-ID: cdi_swepub_primary_oai_slubar_slu_se_66580
Format
–
Schlagworte
1-Acylglycerophosphocholine O-Acyltransferase - chemistry, 1-Acylglycerophosphocholine O-Acyltransferase - genetics, 1-Acylglycerophosphocholine O-Acyltransferase - metabolism, Acyltransferases - chemistry, Acyltransferases - genetics, Acyltransferases - metabolism, Amino Acid Sequence, Arabidopsis, Biochemistry and Molecular Biology, Biokemi och molekylärbiologi, Biomedical and Life Sciences, Crambe, Crambe abyssinica, Crambe Plant - chemistry, Crambe Plant - enzymology, Crambe Plant - genetics, Crambe Plant - metabolism, Erucic Acids - analysis, Erucic Acids - metabolism, Fatty acid composition, Gene Expression Regulation, Plant, Life Sciences, Lipidology, LPCAT, Medical Biochemistry, Medicinal Chemistry, Microbial Genetics and Genomics, Molecular Sequence Data, Neurochemistry, Nutrition, Original Article, PDAT, PDCT, Plant Oils - chemistry, Plant Oils - metabolism, Plants, Genetically Modified - chemistry, Plants, Genetically Modified - enzymology, Plants, Genetically Modified - genetics, Plants, Genetically Modified - metabolism, RNA Interference, RNAi, Seeds - chemistry, Seeds - enzymology, Seeds - genetics, Seeds - metabolism, Sequence Alignment, Transferases (Other Substituted Phosphate Groups) - chemistry, Transferases (Other Substituted Phosphate Groups) - genetics, Transferases (Other Substituted Phosphate Groups) - metabolism

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