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Femtosecond X-ray protein nanocrystallography
Nature (London), 2011-02, Vol.470 (7332), p.73-77
2011

Details

Autor(en) / Beteiligte
Titel
Femtosecond X-ray protein nanocrystallography
Ist Teil von
  • Nature (London), 2011-02, Vol.470 (7332), p.73-77
Ort / Verlag
London: Nature Publishing Group
Erscheinungsjahr
2011
Link zum Volltext
Quelle
EBSCOhost Psychology and Behavioral Sciences Collection
Beschreibungen/Notizen
  • X-ray crystallography provides the vast majority of macromolecular structures, but the success of the method relies on growing crystals of sufficient size. In conventional measurements, the necessary increase in X-ray dose to record data from crystals that are too small leads to extensive damage before a diffraction signal can be recorded. It is particularly challenging to obtain large, well-diffracting crystals of membrane proteins, for which fewer than 300 unique structures have been determined despite their importance in all living cells. Here we present a method for structure determination where single-crystal X-ray diffraction 'snapshots' are collected from a fully hydrated stream of nanocrystals using femtosecond pulses from a hard-X-ray free-electron laser, the Linac Coherent Light Source. We prove this concept with nanocrystals of photosystem I, one of the largest membrane protein complexes. More than 3,000,000 diffraction patterns were collected in this study, and a three-dimensional data set was assembled from individual photosystem I nanocrystals (∼200 nm to 2 μm in size). We mitigate the problem of radiation damage in crystallography by using pulses briefer than the timescale of most damage processes. This offers a new approach to structure determination of macromolecules that do not yield crystals of sufficient size for studies using conventional radiation sources or are particularly sensitive to radiation damage.
Sprache
Englisch
Identifikatoren
ISSN: 0028-0836, 1476-4687
eISSN: 1476-4687
DOI: 10.1038/nature09750
Titel-ID: cdi_swepub_primary_oai_slubar_slu_se_33574
Format
–
Schlagworte
Annan medicinsk grundvetenskap, Atom and Molecular Physics and Optics, Atom- och molekylfysik och optik, bio-inspired, Biologi, Biological and medical sciences, Biology, catalysis (heterogeneous), catalysis (homogeneous), charge transport, chemistry, Crystalline structure, Crystallography, Crystallography, X-Ray - instrumentation, Crystallography, X-Ray - methods, Diffraction, electrodes - solar, Fourier transforms, Fundamental and applied biological sciences. Psychology, Fysiologi, hydrogen and fuel cells, instrumentation, Lasers, materials and chemistry by design, MATERIALS SCIENCE, Methods, Models, Molecular, Molecular, Molecular biophysics, Nanocrystals, Nanoparticles, Nanoparticles - chemistry, NANOSCIENCE AND NANOTECHNOLOGY, Nanotechnology, Nanotechnology - instrumentation, Nanotechnology - methods, NATURAL SCIENCES, NATURVETENSKAP, Other Basic Medicine, photosynthesis (natural and artificial), Photosystem I Protein Complex, Photosystem I Protein Complex - chemistry, Physiology, Protein Conformation, Silicon nitride, solar (fuels), Structure in molecular biology, synthesis (novel materials), synthesis (self-assembly), Time Factors, X-Ray, X-Rays

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