Details

Autor(en) / Beteiligte

• Vetukuri, Ramesh R.
• Kalyandurg, Pruthvi B.
• Saripella, Ganapathi Varma
• Sen, Diya
• Gil, Jose Fernando
• Lukhovitskaya, Nina I.
• Grenville-Briggs, Laura J.
• Savenkov, Eugene I.

Titel

Effect of RNA silencing suppression activity of chrysanthemum virus B p12 protein on small RNA species

Ist Teil von

• Archives of virology, 2020-12, Vol.165 (12), p.2953-2959

Ort / Verlag

Vienna: Springer Vienna

Erscheinungsjahr

2020

Quelle

Springer journals

Beschreibungen/Notizen

• Chrysanthemum virus B encodes a multifunctional p12 protein that acts as a transcriptional activator in the nucleus and as a suppressor of RNA silencing in the cytoplasm. Here, we investigated the impact of p12 on accumulation of major classes of small RNAs (sRNAs). The results show dramatic changes in the sRNA profiles characterised by an overall reduction in sRNA accumulation, changes in the pattern of size distribution of canonical siRNAs and in the ratio between sense and antisense strands, lower abundance of siRNAs with a U residue at the 5′-terminus, and changes in the expression of certain miRNAs, most of which were downregulated.