Details

Autor(en) / Beteiligte

• Apelgren, Peter
• Sämfors, Sanna
• Säljö, Karin
• Mölne, Johan
• Gatenholm, Paul
• Troedsson, Christofer
• Thompson, Eric M.
• Kölby, Lars

Titel

Biomaterial and biocompatibility evaluation of tunicate nanocellulose for tissue engineering

Ist Teil von

• Biomaterials Advances, 2022-06, Vol.137, p.212828-212828, Article 212828

Ort / Verlag

Elsevier B.V

Erscheinungsjahr

2022

Link zum Volltext

Quelle

Alma/SFX Local Collection

Beschreibungen/Notizen

• Extracellular matrix fibril components, such as collagen, are crucial for the structural properties of several tissues and organs. Tunicate-derived cellulose nanofibrils (TNC) combined with living cells could become the next gold standard for cartilage and soft-tissue repair, as TNC fibrils present similar dimensions to collagen, feasible industrial production, and chemically straightforward and cost-efficient extraction procedures. In this study, we characterized the physical properties of TNC derived from aquaculture production in Norwegian fjords and evaluated its biocompatibility regarding induction of an inflammatory response and foreign-body reactions in a Wistar rat model. Additionally, histologic and immunohistochemical analyses were performed for comparison with expanded polytetrafluoroethylene (ePTFE) as a control. The average length of the TNC as determined by atomic force microscopy was tunable from 3 μm to 2.4 μm via selection of a various number of passages through a microfluidizer, and rheologic analysis showed that the TNC hydrogels were highly shear-thinning and with a viscosity dependent on fibril length and concentration. As a bioink, TNC exhibited excellent rheological and printability properties, with constructs capable of being printed with high resolution and fidelity. We found that post-print cross-linking with alginate stabilized the construct shape and texture, which increased its ease of handling during surgery. Moreover, after 30 days in vivo, the constructs showed a highly-preserved shape and fidelity of the grid holes, with these characteristics preserved after 90 days and with no signs of necrosis, infection, acute inflammation, invasion of neutrophil granulocytes, or extensive fibrosis. Furthermore, we observed a moderate foreign-body reaction involving macrophages, lymphocytes, and giant cells in both the TNC constructs and PTFE controls, although TNC was considered a non-irritant biomaterial according to ISO 10993-6 as compared with ePTFE. These findings represent a milestone for future clinical application of TNC scaffolds for tissue repair. One sentence summary: In this study, the mechanical properties of tunicate nanocellulose are superior to nanocellulose extracted from other sources, and the biocompatibility is comparable to that of ePTFE. •In this study, we describe the production, extraction, and preparation of ultrapure tunicate-derived nanocellulose (TNC).•TNC is a new biomaterial with unique fibril dimensions, very high crystallinity, and extreme shear thinning characteristics.•The included biocompatibility evaluation according to ISO standards, reveals that the TNC is a “non-irritant” biomaterial.•The results of this study support the future translation of TNC-based 3D-bioprinted scaffolds to the clinic.