Details

Autor(en) / Beteiligte

• Malina, Carl
• Yu, Rosemary
• Björkeroth, Johan
• Kerkhoven, Eduard J
• Nielsen, Jens

Titel

Adaptations in metabolism and protein translation give rise to the Crabtree effect in yeast

Ist Teil von

• Proceedings of the National Academy of Sciences - PNAS, 2021-12, Vol.118 (51)

Ort / Verlag

United States: National Academy of Sciences

Erscheinungsjahr

2021

Quelle

MEDLINE

Beschreibungen/Notizen

• Aerobic fermentation, also referred to as the Crabtree effect in yeast, is a well-studied phenomenon that allows many eukaryal cells to attain higher growth rates at high glucose availability. Not all yeasts exhibit the Crabtree effect, and it is not known why Crabtree-negative yeasts can grow at rates comparable to Crabtree-positive yeasts. Here, we quantitatively compared two Crabtree-positive yeasts, and , and two Crabtree-negative yeasts, and , cultivated under glucose excess conditions. Combining physiological and proteome quantification with genome-scale metabolic modeling, we found that the two groups differ in energy metabolism and translation efficiency. In Crabtree-positive yeasts, the central carbon metabolism flux and proteome allocation favor a glucose utilization strategy minimizing proteome cost as proteins translation parameters, including ribosomal content and/or efficiency, are lower. Crabtree-negative yeasts, however, use a strategy of maximizing ATP yield, accompanied by higher protein translation parameters. Our analyses provide insight into the underlying reasons for the Crabtree effect, demonstrating a coupling to adaptations in both metabolism and protein translation.