Details

Autor(en) / Beteiligte

• Cuevas-Bermúdez, Abel
• Martínez-Fernández, Verónica
• Garrido-Godino, Ana I.
• Jordán-Pla, Antonio
• Peñate, Xenia
• Martín-Expósito, Manuel
• Gutiérrez, Gabriel
• Govind, Chhabi K.
• Chávez, Sebastián
• Pelechano, Vicent
• Navarro, Francisco

Titel

The association of the RSC remodeler complex with chromatin is influenced by the prefoldin-like Bud27 and determines nucleosome positioning and polyadenylation sites usage in Saccharomyces cerevisiae

Ist Teil von

• Biochimica et biophysica acta. Gene regulatory mechanisms, 2024-03, Vol.1867 (1), p.194995-194995, Article 194995

Ort / Verlag

Netherlands: Elsevier B.V

Erscheinungsjahr

2024

Quelle

MEDLINE

Beschreibungen/Notizen

• The tripartite interaction between the chromatin remodeler complex RSC, RNA polymerase subunit Rpb5 and prefoldin-like Bud27 is necessary for proper RNA pol II elongation. Indeed lack of Bud27 alters this association and affects transcription elongation. This work investigates the consequences of lack of Bud27 on the chromatin association of RSC and RNA pol II, and on nucleosome positioning. Our results demonstrate that RSC binds chromatin in gene bodies and lack of Bud27 alters this association, mainly around polyA sites. This alteration impacts chromatin organization and leads to the accumulation of RNA pol II molecules around polyA sites, likely due to pausing or arrest. Our data suggest that RSC is necessary to maintain chromatin organization around those sites, and any alteration of this organization results in the widespread use of alternative polyA sites. Finally, we also find a similar molecular phenotype that occurs upon TOR inhibition with rapamycin, which suggests that alternative polyadenylation observed upon TOR inhibition is likely Bud27-dependent. •RSC binds chromatin to gene bodies and lack of prefoldin-like Bud27 alters this association, mainly around polyA sites (pAS).•It impacts chromatin organization leading to RNA pol II accumulation around pAS, likely due to pausing or arrest.•Altering RSC-chromatin association results in the widespread use of alternative pAS, impacting alternative polyadenylation.•Our results suggest that alternative polyadenylation observed upon TOR inhibition is influenced by Bud27.