Details

Autor(en) / Beteiligte

• Ståhl, Patrik L.
• Salmén, Fredrik
• Vickovic, Sanja
• Lundmark, Anna
• Navarro, José Fernández
• Magnusson, Jens
• Giacomello, Stefania
• Asp, Michaela
• Westholm, Jakub O.
• Huss, Mikael
• Mollbrink, Annelie
• Linnarsson, Sten
• Codeluppi, Simone
• Borg, Åke
• Pontén, Fredrik
• Costea, Paul Igor
• Sahlén, Pelin
• Mulder, Jan
• Bergmann, Olaf
• Lundeberg, Joakim
• Frisén, Jonas

Titel

Visualization and analysis of gene expression in tissue sections by spatial transcriptomics

Ist Teil von

• Science (American Association for the Advancement of Science), 2016-07, Vol.353 (6294), p.78-82

Ort / Verlag

United States: American Association for the Advancement of Science

Erscheinungsjahr

2016

Quelle

Science Online_科学在线

Beschreibungen/Notizen

• Analysis of the pattern of proteins or messenger RNAs (mRNAs) in histological tissue sections is a cornerstone in biomedical research and diagnostics. This typically involves the visualization of a few proteins or expressed genes at a time. We have devised a strategy, which we call "spatial transcriptomics," that allows visualization and quantitative analysis of the transcriptome with spatial resolution in individual tissue sections. By positioning histological sections on arrayed reverse transcription primers with unique positional barcodes, we demonstrate high-quality RNA-sequencing data with maintained two-dimensional positional information from the mouse brain and human breast cancer. Spatial transcriptomics provides quantitative gene expression data and visualization of the distribution of mRNAs within tissue sections and enables novel types of bioinformatics analyses, valuable in research and diagnostics.