Details

Autor(en) / Beteiligte

• Ryan, Colm J.
• Roguev, Assen
• Patrick, Kristin
• Xu, Jiewei
• Jahari, Harlizawati
• Tong, Zongtian
• Beltrao, Pedro
• Shales, Michael
• Qu, Hong
• Collins, Sean R.
• Kliegman, Joseph I.
• Jiang, Lingli
• Kuo, Dwight
• Tosti, Elena
• Kim, Hyun-Soo
• Edelmann, Winfried
• Keogh, Michael-Christopher
• Greene, Derek
• Tang, Chao
• Cunningham, Pádraig
• Shokat, Kevan M.
• Cagney, Gerard
• Svensson, J. Peter
• Guthrie, Christine
• Espenshade, Peter J.
• Ideker, Trey
• Krogan, Nevan J.

Titel

Hierarchical Modularity and the Evolution of Genetic Interactomes across Species

Ist Teil von

• Molecular cell, 2012-06, Vol.46 (5), p.691-704

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2012

Quelle

Free E-Journal (出版社公開部分のみ）

Beschreibungen/Notizen

• To date, cross-species comparisons of genetic interactomes have been restricted to small or functionally related gene sets, limiting our ability to infer evolutionary trends. To facilitate a more comprehensive analysis, we constructed a genome-scale epistasis map (E-MAP) for the fission yeast Schizosaccharomyces pombe, providing phenotypic signatures for ∼60% of the nonessential genome. Using these signatures, we generated a catalog of 297 functional modules, and we assigned function to 144 previously uncharacterized genes, including mRNA splicing and DNA damage checkpoint factors. Comparison with an integrated genetic interactome from the budding yeast Saccharomyces cerevisiae revealed a hierarchical model for the evolution of genetic interactions, with conservation highest within protein complexes, lower within biological processes, and lowest between distinct biological processes. Despite the large evolutionary distance and extensive rewiring of individual interactions, both networks retain conserved features and display similar levels of functional crosstalk between biological processes, suggesting general design principles of genetic interactomes. [Display omitted] ► Genetic interaction map in fission yeast covering 60% of the nonessential genome ► ∼300 functional modules identified, providing function for uncharacterized genes ► Individual interactions between genes in different processes are poorly conserved ► Overall levels of genetic crosstalk between pairs of processes are highly conserved