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Human beta-defensin-3 promotes wound healing in infected diabetic wounds
The journal of gene medicine, 2009-03, Vol.11 (3), p.220-228
Hirsch, Tobias
Spielmann, Malte
Zuhaili, Baraa
Fossum, Magdalena
Metzig, Marie
Koehler, Till
Steinau, Hans-Ulrich
Yao, Feng
Onderdonk, Andrew Bruce
Steinstraesser, Lars
Eriksson, Elof
2009
Volltextzugriff (PDF)
Details
Autor(en) / Beteiligte
Hirsch, Tobias
Spielmann, Malte
Zuhaili, Baraa
Fossum, Magdalena
Metzig, Marie
Koehler, Till
Steinau, Hans-Ulrich
Yao, Feng
Onderdonk, Andrew Bruce
Steinstraesser, Lars
Eriksson, Elof
Titel
Human beta-defensin-3 promotes wound healing in infected diabetic wounds
Ist Teil von
The journal of gene medicine, 2009-03, Vol.11 (3), p.220-228
Ort / Verlag
Chichester, UK: John Wiley & Sons, Ltd
Erscheinungsjahr
2009
Quelle
Wiley-Blackwell Journals
Beschreibungen/Notizen
Background Infected wounds present a major complication in patients with diabetes. Staphylococcus aureus is the most common single isolate in diabetic wounds. Human beta‐defensin (hBD)‐3 is antimicrobial active and appears to play a key role in the immune response. The present study aimed to analyse the effect of hBD‐3 expression in a model of infected diabetic wounds. Methods Excisional wounds were created on the backs of Yorkshire pigs and Ad5‐CMV‐hBD‐3 vectors were microseeded. Wounds were inoculated with S. aureus, covered with a polyurethane chamber and analysed for transgene expression, bacterial infection, re‐epithelialization, wound contraction, wound fluid production and blood vessel formation. Results hBD‐3‐treated wounds showed a total bacterial load of 2.1 × 108 colony‐forming units (CFU)/g tissue, versus 1.3 × 109 CFU/g tissue for controls (p < 0.001) at day 4. At day 12, no statistical difference could be detected. Re‐epithelialization showed 75 ± 15% wound closure for hBD‐3 expressing wounds and 50 ± 16% for controls (p < 0.01). hBD‐3 expression was in the range 15–20 ng/ml of wound fluid during day 1–4. The lower dose of 2 × 109 Ad5‐CMV‐hBD‐3 showed no effect, suggesting a dose dependency for hBD‐3. Conclusions In the present study, we show that hBD‐3 expression significantly promotes wound closure in S. aureus infected diabetic wounds in a preclinical large‐animal model. Furthermore, a ten‐fold reduction of bacterial growth on day 4 was detected. These findings indicate that beta‐defensin‐3 may play a major role in diabetic wound healing and wound infections. Copyright © 2008 John Wiley & Sons, Ltd.
Sprache
Englisch
Identifikatoren
ISSN: 1099-498X, 1521-2254
eISSN: 1521-2254
DOI: 10.1002/jgm.1287
Titel-ID: cdi_swepub_primary_oai_prod_swepub_kib_ki_se_118429070
Format
–
Schlagworte
Adenoviridae - genetics
,
Adenoviridae - metabolism
,
Animals
,
bacterial infection
,
beta-Defensins - genetics
,
beta-Defensins - metabolism
,
Cells, Cultured
,
Diabetes Complications - physiopathology
,
Diabetes Complications - therapy
,
Diabetes Mellitus
,
Gene therapy
,
gene transfer
,
Gene Transfer Techniques
,
Genetic Therapy
,
Genetic Vectors
,
host defense peptides
,
Humans
,
innate immunity
,
Keratinocytes - cytology
,
Keratinocytes - physiology
,
Medicin och hälsovetenskap
,
Neovascularization, Physiologic
,
S. aureus
,
Staphylococcal Skin Infections - microbiology
,
Staphylococcal Skin Infections - physiopathology
,
Staphylococcal Skin Infections - therapy
,
Staphylococcus aureus
,
Staphylococcus aureus - metabolism
,
Swine
,
Transgenes
,
Wound Healing
,
Wound Infection - microbiology
,
Wound Infection - physiopathology
,
Wound Infection - therapy
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