Details

Autor(en) / Beteiligte

• Moralejo, Daniel H.
• Fuller, Jessica M.
• Rutledge, Elizabeth A.
• Van Yserloo, Brian
• Ettinger, Ruth A.
• Jensen, Richard
• Osborne, William
• Kwitek, Anne
• Lernmark, Åke

Titel

BB rat Gimap gene expression in sorted lymphoid T and B cells

Ist Teil von

• Life sciences (1973), 2011-11, Vol.89 (19), p.748-754

Ort / Verlag

Netherlands: Elsevier Inc

Erscheinungsjahr

2011

Quelle

MEDLINE

Beschreibungen/Notizen

• The Gimap gene family has been shown to be integral to T cell survival and development. A frameshift mutation in Gimap5, one of seven members of the Gimap family, results in lymphopenia and is a prerequisite for spontaneous type 1 diabetes (T1D) in the BioBreeding (BB) rat. While not contributing to lymphopenia, the Gimap family members proximal to Gimap5, encompassed within the Iddm39 quantitative trait locus (QTL), have been implicated in T1D. We hypothesized that expression of the Gimap family members within the Iddm39 QTL, during thymocyte development as well as in peripheral T and B cells contribute to T1D. Cell sorted subpopulations were analyzed by quantitative real time (qRT) PCR. Gimap4 expression was reduced in DR. lyp/lyp rat double negative, double positive and CD8 single positive (SP) thymocytes while expression of Gimap8, Gimap6, and Gimap7 was reduced only in CD8 SP thymocytes. Interestingly, expression of the entire Gimap gene family was reduced in DR. lyp/lyp rat peripheral T cells compared to non-lymphopenic, non-diabetic DR. +/+ rats. With the exception of Gimap6, the Gimap family genes were not expressed in B cells from spleen and mesenteric lymph node (MLN). Expression of Gimap9 was only detected in hematopoietic cells of non B cell lineage such as macrophage, dendritic or NK cells. These results suggest that lack of the Gimap5 protein in the DR. lyp/lyp congenic rat was associated with impaired expression of the entire family of Gimap genes and may regulate T cell homeostasis in the peripheral lymphoid organs.