Vaccine, 2007-12, Vol.25 (51), p.8598-8610
2007
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- Autor(en) / Beteiligte
- Titel
- Sublingual immunization induces broad-based systemic and mucosal immune responses in mice
- Ist Teil von
- Vaccine, 2007-12, Vol.25 (51), p.8598-8610
- Ort / Verlag
- Oxford: Elsevier Ltd
- Erscheinungsjahr
- 2007
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Abstract The potential of sublingual (s.l.) delivery of vaccine was examined in mice. We show the existence of a dense network of dendritic cells (DCs) in the s.l. epithelium and a rapid and transient increase in the frequency of s.l. DCs after topical application of cholera toxin (CT) adjuvant under the tongue. S.l. immunization with ovalbumin and CT induced vigorous systemic and mucosal antibody responses. Such treatment promoted mixed Th1 and Th2 cytokine responses and induced cytotoxic CD8+ T cells in lung tissues and in systemic lymphoid organs. S.l. immunization was comparable to intranasal immunization and was superior to oral immunization regarding the magnitude and anatomic dissemination of the induced immune responses. S.l. administration of live influenza virus at a dose lethal by the nasal route was well tolerated and did not redirect virus to the olfactory bulb. These features underscore the potential of the s.l. mucosa to serve as an alternative vaccine delivery route.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 0264-410XeISSN: 1873-2518DOI: 10.1016/j.vaccine.2007.09.073Titel-ID: cdi_swepub_primary_oai_gup_ub_gu_se_85337
- Format
- –
- Schlagworte
- Adenoviruses, Adjuvants, Adjuvants, Immunologic, Administration, Administration, Sublingual, Adoptive Transfer, Allergy and Immunology, analysis, Animals, Antibodies, Antibodies, Viral - analysis, Antibodies, Viral - biosynthesis, Antibody-Producing Cells, Antibody-secreting cells, Antigens, Applied microbiology, Bacterial diseases, Bacteriology, Biological and medical sciences, biosynthesis, CD4-Positive T-Lymphocytes, CD4-Positive T-Lymphocytes - immunology, CD8-Positive T-Lymphocytes, CD8-Positive T-Lymphocytes - immunology, Cholera, Cholera Toxin, Cholera Toxin - immunology, Cytokines, Cytokines - biosynthesis, cytology, Cytotoxic T lymphocytes, Dendritic Cells, Dendritic Cells - immunology, Dose-Response Relationship, Dose-Response Relationship, Immunologic, Experiments, Female, Flow Cytometry, Fundamental and applied biological sciences. Psychology, Human bacterial diseases, Immune system, Immunity, Immunity, Mucosal - immunology, Immunization, Immunization - methods, Immunohistochemistry, Immunologic, immunology, Inbred BALB C, Inbred C57BL, Infectious diseases, Influenza, Influenza virus, Laboratories, Medical sciences, methods, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Microbiology, Microbiology in the medical area, Mikrobiologi inom det medicinska området, Miscellaneous, Molecular weight, Mouth Mucosa, Mouth Mucosa - cytology, Mouth Mucosa - immunology, Mucosal, Mucosal vaccine, Ovalbumin, Ovalbumin - immunology, Reverse Transcriptase Polymerase Chain Reaction, Rodents, Small intestine, Studies, Sublingual, Sublingual mucosa, Tropical bacterial diseases, Vaccines, Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects), Viral, Viruses