Details

Autor(en) / Beteiligte

• Panyard, Daniel J.
• McKetney, Justin
• Deming, Yuetiva K.
• Morrow, Autumn R.
• Ennis, Gilda E.
• Jonaitis, Erin M.
• Van Hulle, Carol A.
• Yang, Chengran
• Sung, Yun Ju
• Ali, Muhammad
• Kollmorgen, Gwendlyn
• Suridjan, Ivonne
• Bayfield, Anna
• Bendlin, Barbara B.
• Zetterberg, Henrik
• Blennow, Kaj
• Cruchaga, Carlos
• Carlsson, Cynthia M.
• Johnson, Sterling C.
• Asthana, Sanjay
• Coon, Joshua J.
• Engelman, Corinne D.

Titel

Large‐scale proteome and metabolome analysis of CSF implicates altered glucose and carbon metabolism and succinylcarnitine in Alzheimer's disease

Ist Teil von

• Alzheimer's & dementia, 2023-12, Vol.19 (12), p.5447-5470

Ort / Verlag

United States

Erscheinungsjahr

2023

Quelle

Wiley-Blackwell Journals

Beschreibungen/Notizen

• INTRODUCTION A hallmark of Alzheimer's disease (AD) is the aggregation of proteins (amyloid beta [A] and hyperphosphorylated tau [T]) in the brain, making cerebrospinal fluid (CSF) proteins of particular interest. METHODS We conducted a CSF proteome‐wide analysis among participants of varying AT pathology (n = 137 participants; 915 proteins) with nine CSF biomarkers of neurodegeneration and neuroinflammation. RESULTS We identified 61 proteins significantly associated with the AT category (P < 5.46 × 10−5) and 636 significant protein‐biomarker associations (P < 6.07 × 10−6). Proteins from glucose and carbon metabolism pathways were enriched among amyloid‐ and tau‐associated proteins, including malate dehydrogenase and aldolase A, whose associations with tau were replicated in an independent cohort (n = 717). CSF metabolomics identified and replicated an association of succinylcarnitine with phosphorylated tau and other biomarkers. DISCUSSION These results implicate glucose and carbon metabolic dysregulation and increased CSF succinylcarnitine levels with amyloid and tau pathology in AD. Highlights Cerebrospinal fluid (CSF) proteome enriched for extracellular, neuronal, immune, and protein processing. Glucose/carbon metabolic pathways enriched among amyloid/tau‐associated proteins. Key glucose/carbon metabolism protein associations independently replicated. CSF proteome outperformed other omics data in predicting amyloid/tau positivity. CSF metabolomics identified and replicated a succinylcarnitine–phosphorylated tau association.