Details

Autor(en) / Beteiligte

• Roodgar, Morteza
• Suchy, Fabian P.
• Nguyen, Lan H.
• Bajpai, Vivek K.
• Sinha, Rahul
• Vilches-Moure, Jose G.
• Van Bortle, Kevin
• Bhadury, Joydeep
• Metwally, Ahmed
• Jiang, Lihua
• Jian, Ruiqi
• Chiang, Rosaria
• Oikonomopoulos, Angelos
• Wu, Joseph C.
• Weissman, Irving L.
• Mankowski, Joseph L.
• Holmes, Susan
• Loh, Kyle M.
• Nakauchi, Hiromitsu
• VandeVoort, Catherine A.
• Snyder, Michael P.

Titel

Chimpanzee and pig-tailed macaque iPSCs: Improved culture and generation of primate cross-species embryos

Ist Teil von

• Cell reports (Cambridge), 2022-08, Vol.40 (9), p.111264-111264, Article 111264

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2022

Quelle

MEDLINE

Beschreibungen/Notizen

• As our closest living relatives, non-human primates uniquely enable explorations of human health, disease, development, and evolution. Considerable effort has thus been devoted to generating induced pluripotent stem cells (iPSCs) from multiple non-human primate species. Here, we establish improved culture methods for chimpanzee (Pan troglodytes) and pig-tailed macaque (Macaca nemestrina) iPSCs. Such iPSCs spontaneously differentiate in conventional culture conditions, but can be readily propagated by inhibiting endogenous WNT signaling. As a unique functional test of these iPSCs, we injected them into the pre-implantation embryos of another non-human species, rhesus macaques (Macaca mulatta). Ectopic expression of gene BCL2 enhances the survival and proliferation of chimpanzee and pig-tailed macaque iPSCs within the pre-implantation embryo, although the identity and long-term contribution of the transplanted cells warrants further investigation. In summary, we disclose transcriptomic and proteomic data, cell lines, and cell culture resources that may be broadly enabling for non-human primate iPSCs research. [Display omitted] •Inhibiting WNT signaling improves culture of chimp and pig-tailed macaque iPSCs•Endogenous high expression of BCL2 in the rhesus inner cell mass•Expression of BCL2 enhances proliferation of chimp iPSCs in rhesus early embryo•Co-culture of chimp and human iPSCs can differentiate into integrated cardiomyocytes Roodgar et al. demonstrate an improved culture of chimpanzee and pig-tailed macaque iPSCs. They show high expression of endogenous BCL2 in rhesus macaque ICM. Through over expression of BCL2 in chimpanzee iPSCs, they generate primate cross-species pre-implantation embryos composed of chimpanzee iPSCs and rhesus pre-implantation embryos.