Details

Autor(en) / Beteiligte

• Madunić, Katarina
• Zhang, Tao
• Mayboroda, Oleg A.
• Holst, Stephanie
• Stavenhagen, Kathrin
• Jin, Chunsheng
• Karlsson, Niclas G.
• Lageveen-Kammeijer, Guinevere S. M.
• Wuhrer, Manfred

Titel

Colorectal cancer cell lines show striking diversity of their O-glycome reflecting the cellular differentiation phenotype

Ist Teil von

• Cellular and molecular life sciences : CMLS, 2021-01, Vol.78 (1), p.337-350

Ort / Verlag

Cham: Springer International Publishing

Erscheinungsjahr

2021

Quelle

SpringerLink

Beschreibungen/Notizen

• Alterations in protein glycosylation in colorectal cancer (CRC) have been extensively studied using cell lines as models. However, little is known about their O -glycome and the differences in glycan biosynthesis in different cell types. To provide a better understanding of the variation in O -glycosylation phenotypes and their association with other molecular features, an in-depth O- glycosylation analysis of 26 different CRC cell lines was performed. The released O- glycans were analysed on porous graphitized carbon nano-liquid chromatography system coupled to a mass spectrometer via electrospray ionization (PGC-nano-LC–ESI-MS/MS) allowing isomeric separation as well as in-depth structural characterization. Associations between the observed glycan phenotypes with previously reported cell line transcriptome signatures were examined by canonical correlation analysis. Striking differences are observed between the O- glycomes of 26 CRC cell lines. Unsupervized principal component analysis reveals a separation between well-differentiated colon-like and undifferentiated cell lines. Colon-like cell lines are characterized by a prevalence of I-branched and sialyl Lewis x/a epitope carrying glycans, while most undifferentiated cell lines show absence of Lewis epitope expression resulting in dominance of truncated α2,6-core sialylated glycans. Moreover, the expression of glycan signatures associates with the expression of glycosyltransferases that are involved in their biosynthesis, providing a deeper insight into the regulation of glycan biosynthesis in different cell types. This untargeted in-depth screening of cell line O -glycomes paves the way for future studies exploring the role of glycosylation in CRC development and drug response leading to discovery of novel targets for the development of anti-cancer antibodies.