Details

Autor(en) / Beteiligte

• Jalouli, Miranda-Masoumeh
• Jalouli, Jamshid
• Hasséus, Bengt
• Öhman, Jenny
• Hirsch, Jan-Michaél
• Sand, Lars

Titel

Nested PCR for detection of HSV-1 in oral mucosa

Ist Teil von

• Medicina oral, patología oral y cirugía bucal, 2015-11, Vol.20 (6), p.e664-e669

Ort / Verlag

Spain: Medicina Oral S.L

Erscheinungsjahr

2015

Quelle

MEDLINE

Beschreibungen/Notizen

• It has been estimated that 15%-20% of human tumours are driven by infection and inflammation, and viral infections play an important role in malignant transformation. The evidence that herpes simplex virus type 1 (HSV-1) could be involved in the aetiology of oral cancer varies from weak to persuasive. This study aimed to investigate by nested PCR (NPCR) the prevalence of HSV-1 in samples from normal oral mucosa, oral leukoplakia, and oral squamous cell carcinoma (OSCC). We investigated the prevalence of HSV-1 in biopsies obtained from 26 fresh, normal oral mucosa from healthy volunteers as well as 53 oral leukoplakia and 27 OSCC paraffin-embedded samples. DNA was extracted from the specimens and investigated for the presence of HSV-1 by nested polymerase chain reaction (NPCR) and DNA sequencing. HSV-1 was detected in 14 (54%) of the healthy samples, in 19 (36%) of the oral leukoplakia samples, and in 14 (52%) of the OSCC samples. The differences were not statistically significant. We observed a high incidence of HSV-1 in healthy oral mucosa, oral leukoplakia, and OSCC tissues. Thus, no connection between OSCC development and presence of HSV-1 was detected.