Details

Autor(en) / Beteiligte

• Barone, Angela
• Säljö, Karin
• Benktander, John
• Blomqvist, Maria
• Månsson, Jan-Eric
• Johansson, Bengt R.
• Mölne, Johan
• Aspegren, Anders
• Björquist, Petter
• Breimer, Michael E.
• Teneberg, Susann

Titel

Sialyl-lactotetra, a Novel Cell Surface Marker of Undifferentiated Human Pluripotent Stem Cells

Ist Teil von

• The Journal of biological chemistry, 2014-07, Vol.289 (27), p.18846-18859

Ort / Verlag

United States: Elsevier Inc

Erscheinungsjahr

2014

Quelle

Electronic Journals Library

Beschreibungen/Notizen

• Cell surface glycoconjugates are used as markers for undifferentiated pluripotent stem cells. Here, antibody binding and mass spectrometry characterization of acid glycosphingolipids isolated from a large number (1 × 109 cells) of human embryonic stem cell (hESC) lines allowed identification of several novel acid glycosphingolipids, like the gangliosides sialyl-lactotetraosylceramide and sialyl-globotetraosylceramide, and the sulfated glycosphingolipids sulfatide, sulf-lactosylceramide, and sulf-globopentaosylceramide. A high cell surface expression of sialyl-lactotetra on hESC and human induced pluripotent stem cells (hiPSC) was demonstrated by flow cytometry, immunohistochemistry, and electron microscopy, whereas sulfated glycosphingolipids were only found in intracellular compartments. Immunohistochemistry showed distinct cell surface anti-sialyl-lactotetra staining on all seven hESC lines and three hiPSC lines analyzed, whereas no staining of hESC-derived hepatocyte-like or cardiomyocyte-like cells was obtained. Upon differentiation of hiPSC into hepatocyte-like cells, the sialyl-lactotetra epitope was rapidly down-regulated and not detectable after 14 days. These findings identify sialyl-lactotetra as a promising marker of undifferentiated human pluripotent stem cells. Background: Carbohydrate epitopes are often used as markers for characterization of human pluripotent stem cells (hPSC). Results: Sialyl-lactotetra is highly expressed on the cell surface of hPSC lines, and the expression decreased upon early differentiation. Conclusion: Sialyl-lactotetra is a novel marker of undifferentiated human stem cells. Significance: These findings might impact future applications in regenerative medicine.