Details

Autor(en) / Beteiligte

• Asp, Lennart
• Kartberg, Fredrik
• Fernandez-Rodriguez, Julia
• Smedh, Maria
• Elsner, Markus
• Laporte, Frederic
• Bárcena, Montserrat
• Jansen, Karen A
• Valentijn, Jack A
• Koster, Abraham J
• Bergeron, John J M
• Nilsson, Tommy
• Munro, Sean

Titel

Early stages of Golgi vesicle and tubule formation require diacylglycerol

Ist Teil von

• Molecular biology of the cell, 2009-02, Vol.20 (3), p.780-790

Ort / Verlag

United States: The American Society for Cell Biology

Erscheinungsjahr

2009

Link zum Volltext

Quelle

MEDLINE

Beschreibungen/Notizen

• We have investigated the role for diacylglycerol (DAG) in membrane bud formation in the Golgi apparatus. Addition of propranolol to specifically inhibit phosphatidate phosphohydrolase (PAP), an enzyme responsible for converting phosphatidic acid into DAG, effectively prevents formation of membrane buds. The effect of PAP inhibition on Golgi membranes is rapid and occurs within 3 min. Removal of the PAP inhibitor then results in a rapid burst of buds, vesicles, and tubules that peaks within 2 min. The inability to form buds in the presence of propranolol does not appear to be correlated with a loss of ARFGAP1 from Golgi membranes, as knockdown of ARFGAP1 by RNA interference has little or no effect on actual bud formation. Rather, knockdown of ARFGAP1 results in an increase in membrane buds and a decrease of vesicles and tubules suggesting it functions in the late stages of scission. How DAG promotes bud formation is discussed.