Nature medicine, 2009-11, Vol.15 (11), p.1273-1280
2009
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- Autor(en) / Beteiligte
- Titel
- Dual roles for hepatic lectin receptors in the clearance of chilled platelets
- Ist Teil von
- Nature medicine, 2009-11, Vol.15 (11), p.1273-1280
- Ort / Verlag
- United States: Nature Publishing Group
- Erscheinungsjahr
- 2009
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Rapid chilling causes glycoprotein-Ib (GPIb) receptors to cluster on blood platelets. Hepatic macrophage beta(2) integrin binding to beta-N-acetylglucosamine (beta-GlcNAc) residues in the clusters leads to rapid clearance of acutely chilled platelets after transfusion. Although capping the beta-GlcNAc moieties by galactosylation prevents clearance of short-term-cooled platelets, this strategy is ineffective after prolonged refrigeration. We report here that prolonged refrigeration increased the density and concentration of exposed galactose residues on platelets such that hepatocytes, through Ashwell-Morell receptor binding, become increasingly involved in platelet removal. Macrophages rapidly removed a large fraction of transfused platelets independent of their storage conditions. With prolonged platelet chilling, hepatocyte-dependent clearance further diminishes platelet recovery and survival after transfusion. Inhibition of chilled platelet clearance by both beta(2) integrin and Ashwell-Morell receptors may afford a potentially simple method for storing platelets in the cold.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1078-8956, 1546-170XeISSN: 1546-170XDOI: 10.1038/nm.2030Titel-ID: cdi_swepub_primary_oai_gup_ub_gu_se_103581
- Format
- –
- Schlagworte
- Acetylglucosamine - metabolism, Acetylglucosamine - pharmacology, alpha-Fetoproteins - pharmacology, Animals, Annan klinisk medicin, Ashwell-Morell receptor, Asialoglycoprotein Receptor - metabolism, Asialoglycoproteins - pharmacology, Blood Component Removal, Blood platelets, Blood Platelets - cytology, Blood Platelets - drug effects, Blood Platelets - physiology, Blood Preservation - methods, Blood products, Blood Transfusion - methods, Blood transfusions, Carbohydrate Conformation, CD18 Antigens - metabolism, Cell and Molecular Biology, Cell Line, Transformed, Cell- och molekylärbiologi, Cellular biology, Chemical properties, clearance, Cold Temperature, Flow Cytometry, Galactose - metabolism, Glycoproteins, Glycosylation, Humans, lectin, Lectins, Macrophages, Macrophages - drug effects, Macrophages - physiology, Methods, Mice, Other Clinical Medicine, Peptide Fragments - pharmacology, Phagocytes - drug effects, Physiological aspects, Platelet Glycoprotein GPIb-IX Complex - metabolism, platelets, Protein Binding - drug effects, Protein Binding - physiology, Refrigeration, Refrigeration - methods, sialic acid, Storage, Time Factors, Transfusion