Details

Autor(en) / Beteiligte

• Lönn, Peter
• Landegren, Ulf

Titel

Close Encounters – Probing Proximal Proteins in Live or Fixed Cells

Ist Teil von

• Trends in biochemical sciences (Amsterdam. Regular ed.), 2017-07, Vol.42 (7), p.504-515

Ort / Verlag

England: Elsevier Ltd

Erscheinungsjahr

2017

Quelle

MEDLINE

Beschreibungen/Notizen

• The well-oiled machinery of the cellular proteome operates via variable expression, modifications, and interactions of proteins, relaying genomic and transcriptomic information to coordinate cellular functions. In recent years, a number of techniques have emerged that serve to identify sets of proteins acting in close proximity in the course of orchestrating cellular activities. These proximity-dependent assays, including BiFC, BioID, APEX, FRET, and isPLA, have opened up new avenues to examine protein interactions in live or fixed cells. We review herein the current status of proximity-dependent in situ techniques. We compare the advantages and limitations of the methods, underlining recent progress and the growing importance of these techniques in basic research, and we discuss their potential as tools for drug development and diagnostics. Proximity-dependent protein analyses identify target proteins that are in close proximity of each other. This is increasingly used to study sets of proteins that function together to coordinate cellular processes. The proximity-dependent methods BiFC, BioID, APEX, FRET, and isPLA provide means to examine protein–protein interactions in situ in living or fixed cells. Applications of proximity-dependent assays in situ extend from preclinical research in genetically modified cell cultures and in vivo models to studies of clinically relevant biopsy material for drug development and diagnostics.