Genome Biology, 2016-09, Vol.17 (1), p.188-188, Article 188
2016
Volltextzugriff (PDF)
Details
- Autor(en) / Beteiligte
- Titel
- Multiplexed, targeted profiling of single-cell proteomes and transcriptomes in a single reaction
- Ist Teil von
- Genome Biology, 2016-09, Vol.17 (1), p.188-188, Article 188
- Ort / Verlag
- England: BioMed Central
- Erscheinungsjahr
- 2016
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- We present a scalable, integrated strategy for coupled protein and RNA detection from single cells. Our approach leverages the DNA polymerase activity of reverse transcriptase to simultaneously perform proximity extension assays and complementary DNA synthesis in the same reaction. Using the Fluidigm C1™ system, we profile the transcriptomic and proteomic response of a human breast adenocarcinoma cell line to a chemical perturbation, benchmarking against in situ hybridizations and immunofluorescence staining, as well as recombinant proteins, ERCC Spike-Ins, and population lysate dilutions. Through supervised and unsupervised analyses, we demonstrate synergies enabled by simultaneous measurement of single-cell protein and RNA abundances. Collectively, our generalizable approach highlights the potential for molecular metadata to inform highly-multiplexed single-cell analyses.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1474-760X, 1474-7596, 1465-6906eISSN: 1474-760XDOI: 10.1186/s13059-016-1045-6Titel-ID: cdi_swepub_primary_oai_DiVA_org_uu_305451
- Format
- –
- Schlagworte
- Adenocarcinoma, Antigens, Apoptosis, Bioinformatics, Breast Neoplasms - genetics, Breast Neoplasms - pathology, Cell cycle, Complementary DNA, Deoxyribonucleic acid, DNA, DNA biosynthesis, DNA polymerase, DNA-directed DNA polymerase, Female, Gene expression, Gene Expression Profiling, Humans, Immunofluorescence, Immunoglobulins, Kinases, Metadata, Method, Methods, Proteins, Proteome - genetics, Proteomics, Proximity extension assay, Ribonucleic acid, RNA, RNA - biosynthesis, RNA - genetics, RNA-directed DNA polymerase, Single-Cell Analysis, Single-cell multi-omics, Single-cell protein, Single-cell proteomics, Single-cell transcriptomics, Transcriptome - genetics