Molecular therapy, 2012-05, Vol.20 (5), p.960-971
2012
Details
- Autor(en) / Beteiligte
- Titel
- Microvesicle-associated AAV Vector as a Novel Gene Delivery System
- Ist Teil von
- Molecular therapy, 2012-05, Vol.20 (5), p.960-971
- Ort / Verlag
- United States: Elsevier Inc
- Erscheinungsjahr
- 2012
- Link zum Volltext
- Quelle
- MEDLINE
- Beschreibungen/Notizen
- Adeno-associated virus (AAV) vectors have shown remarkable efficiency for gene delivery to cultured cells and in animal models of human disease. However, limitations to AAV vectored gene transfer exist after intravenous transfer, including off-target gene delivery (e.g., liver) and low transduction of target tissue. Here, we show that during production, a fraction of AAV vectors are associated with microvesicles/exosomes, termed vexosomes (vector-exosomes). AAV capsids associated with the surface and in the interior of microvesicles were visualized using electron microscopy. In cultured cells, vexosomes outperformed conventionally purified AAV vectors in transduction efficiency. We found that purified vexosomes were more resistant to a neutralizing anti-AAV antibody compared to conventionally purified AAV. Finally, we show that vexosomes bound to magnetic beads can be attracted to a magnetized area in cultured cells. Vexosomes represent a unique entity which offers a promising strategy to improve gene delivery.
- Sprache
- Englisch
- Identifikatoren
- ISSN: 1525-0016, 1525-0024eISSN: 1525-0024DOI: 10.1038/mt.2011.303Titel-ID: cdi_swepub_primary_oai_DiVA_org_umu_55673
- Format
- –
- Schlagworte
- Adeno-associated virus, Antibodies, Capsid - chemistry, Capsid - ultrastructure, Cell Line, Tumor, Cells, Centrifugation, Density Gradient, Clinical trials, Dependovirus - genetics, Dependovirus - ultrastructure, Gene therapy, Gene Transfer Techniques, Genetic Therapy - methods, Genetic Vectors - ultrastructure, Humans, Labeling, Medical schools, Microscopy, Electron, Neoplasms - genetics, Neoplasms - therapy, Neurosciences, Original, Plasmids, Transduction, Genetic, Transfection, Transmission electron microscopy, Vectors (Biology)