Details

Autor(en) / Beteiligte

• Vilas, Jose Luis
• Gómez-Blanco, Josué
• Conesa, Pablo
• Melero, Roberto
• Miguel de la Rosa-Trevín, José
• Otón, Joaquin
• Cuenca, Jesús
• Marabini, Roberto
• Carazo, José María
• Vargas, Javier
• Sorzano, Carlos Oscar S.

Titel

MonoRes: Automatic and Accurate Estimation of Local Resolution for Electron Microscopy Maps

Ist Teil von

• Structure (London), 2018-02, Vol.26 (2), p.337-344.e4

Ort / Verlag

United States: Elsevier Ltd

Erscheinungsjahr

2018

Link zum Volltext

Quelle

Elsevier ScienceDirect Journals

Beschreibungen/Notizen

• Since the beginning of electron microscopy, resolution has been a critical parameter. In this article, we propose a fully automatic, accurate method for determining the local resolution of a 3D map (MonoRes). The foundation of this algorithm is an extension of the concept of analytic signal, termed monogenic signal. The map is filtered at different frequencies and the amplitude of the monogenic signal is calculated, after which a criterion is applied to determine the resolution at each voxel. MonoRes is fully automatic without compulsory user parameters, with great accuracy in all tests, and is computationally more rapid than existing methods in the field. In addition, MonoRes offers the option of local filtering of the original map based on the calculated local resolution. [Display omitted] •Resolution is a local feature of electron density maps•MonoRes provides a fast, automatic, and accurate estimation of resolution maps•Local filtering of electron density maps is provided•MonoRes method is invariant under b-factor and prewhitening correction Vilas et al. present MonoRes, a fast and fully automatic method to determine the local resolution in electron density maps. It also allows local filtering of the original map at local resolution values.