Details

Autor(en) / Beteiligte

• H Lundqvist-Gustafsson
• T Bengtsson

Titel

Activation of the granule pool of the NADPH oxidase accelerates apoptosis in human neutrophils

Ist Teil von

• Journal of leukocyte biology, 1999-02, Vol.65 (2), p.196-204

Ort / Verlag

United States: Society for Leukocyte Biology

Erscheinungsjahr

1999

Quelle

Wiley Online Library - AutoHoldings Journals

Beschreibungen/Notizen

• Oxidative stress induces apoptosis in many types of cells, including human neutrophils. Our objective was to determine whether reactive oxygen species (ROS) produced by activated neutrophils are associated with accelerated apoptosis. Exposing neutrophils to ionomycin or phorbol myristate acetate (PMA) induced intracellular H2O2 production and rapid onset of apoptosis, measured as condensed chromatin, cellular shrinkage, and DNA fragmentation. Neutrophils activated with formyl‐methionyl‐leucyl‐phenylalanine (fMLP) generated mainly extracellular H2O2 and did not undergo apoptosis. Exogenously added H2O2, together with the catalase blocker sodium azide, induced apoptosis to the same extent and with similar kinetics as PMA and ionomycin. Adenosine inhibited ionomycin‐induced intracellular H2O2 production and apoptosis. Neither PMA nor ionomycin caused apoptosis in dimethyl sulfoxide‐differentiated HL‐60 cells, which are incapable of intracellular H2O2 production, whereas H2O2 induced apoptosis more efficiently in these cells than in neutrophils. We propose that activated neutrophils use intracellularly formed H2O2 to commit suicide. J. Leukoc. Biol. 65: 196–204; 1999.